NF—κB、VEGF在非小细胞肺癌中的表达及其与肿瘤血管形成的研究

目的探讨核因子-κB（NF-κB）和血管内皮生长因子（VEGF）在非小细胞肺癌（NSCLC）中的表达及其与肿瘤血管形成的关系。方法应用免疫组织化学方法，检测56例非小细胞肺癌及20例癌旁肺组织中的NF-κBP65、VEGF的表达，并用抗CD34测定肿瘤血管的密度（MVD）。结果（1）在非小细胞肺癌中NF-κBP65、VEGF的表达阳性率分别为83．9％（47／56）、69．6％（39／56），明显高于癌旁组织（P〈O．05）；（2）NF-κBP65的表达在不同的TNM分期、淋巴结及胸腔积液、吸烟的分组之间差异有统计学意义，VEGF的表达在淋巴结及胸膜转移之间差异有统计学意义；（3）NF-κBP65、VEGF、MVD三者间存在明显相关性。结论NF-κB、VEGF异常表达与NSCLC的发生、发展及肿瘤血管的形成有着密切的关系。

Expression of NF-κB and VEGF in non-small cell lung cancer and its relationships with tumor angiogenesis

Objective To evaluate the expression of vascular endothelial growth factor（VEGF）,and NF-κB, and its correlation with tumor angiogenesis in non-small cell lung cancer（NSCLC）. Methods Expressions of NF-κB P65 and VEGF in NSCLC（n=56）and benign tumor tissue（n=20） were detected by immunohistoehemical staining, a monoclonal antibody directed against CD34 to identi{y intratumoral mierovessel density （iMVD）. Results The positive rates of NF-κB P65 and VEGF expressions in NSCLC tissue were 83.9% ,and 69.6% respectively significantly higher than those of benign tumor tissue （P〈0.05）. The expression level of NF-κB P65 was related to tumor stages, lymphnode involvement, pleural metastasis and smoking of patients （P〈0.05）, while the expression level of VEGF was related to lymphnode involvement and pleural metastasis. There was a statistically significant correlation between expressions of NF-κB P65 and VEGF, NF-κB P65 and MVD, VEGF and MVD, respectively （P 〈 0. 05）. Conclusion The high expressions of NF-κB P65 and VEGF in NSCLC tissues may be related to tumor invasion and angiogenesis.