SRAP分子标记技术分析鱼腥草居群的遗传多样性

在正交设计对鱼腥草SRAP—PcR反应体系进行优化的基础上，分析鱼腥草居群的遗传多样性的结果表明：最佳的SRAP-PCR反应体系为每10μL溶液中含有0．2mmol·L-1 dNTPs、20ng模板DNA、30ng·μL -1引物、0．5UTaq聚合酶和2mmol·L-1 MgCl2；最佳的复性温度和循环次数为53℃和35次；从340个引物组合中筛选出条带清晰、多态性好的118个引物组合，并扩增出7582个谱带，多态性谱带有6590个，多态率为86．92％；在这些谱带中发现19条特异性的谱带，其中居群ZY42占31．58％；聚类分析结果显示鱼腥草居群存在非常丰富的遗传变异。

An Analysis of Genetic Diversity of Houttuynia cordata Thunb. Population by SRAP Molecular Markers

On the basis of optimized orthogonally SRAP-PCR amplification system, genetic diversity of Houttuynia cordata population was studied. The results showed that the optimum SRAP-PCR reaction system contained 0.2 mmol.L-1 dNTPs, 20 ng template DNA, 30 ng.μL-1 primers, 0.5 U Taq DNA polymerase and 2 mmol.L-1 MgC;2 in a total volume of 10μL and the optimal annealing temperature and cycling times was 53 ℃ and 35 times. 118 effective primer-combinations were screened from 340 primer-combinations. A total of 7 582 bands were detected with those primers and 6 590 of them were polymorphic. The percentage of polymorphy was 86.92%. Among those bands, 19 bands had specificity and ZY42 population was 31.58%. The cluster results showed that the genetic differentiation was very abundant, and the SRAP molecular marker was used to identify the genetic differences of H. cordata population.