|
|||||
|
|
| Geotrichum sp．SYBC WU-3脂肪酶的双水相萃取和酶学性质 | |
| 引用本文： | 王蕾,蔡宇杰,廖祥儒,张峰,张大兵.Geotrichum sp．SYBC WU-3脂肪酶的双水相萃取和酶学性质[J].生物加工过程,2009,7(2):57-62. |
| 作者姓名： | 王蕾 蔡宇杰 廖祥儒 张峰 张大兵 |
| 作者单位： | 1. 江南大学,生物工程学院,工业生物技术教育部重点实验室,无锡,214122;内蒙古科技大学,生物与化学工程学院,包头,014010 2. 江南大学,生物工程学院,工业生物技术教育部重点实验室,无锡,214122 3. 江苏汉邦科技有限公司,淮安,223001 |
| 基金项目： | 江南大学人才引进基金 |
| 摘 要： | 初步研究双水相体系对Geotrichum sp．SYBC WU-3脂肪酶的萃取分离效果，选用PEC4000／NaH2 PO4作为戍相系统进行系统研究，考察影响脂肪酶萃取的各种因素（如PEG相对分子质量及质量分数、NaH2PO4质量浓度、pH），并采用正交实验进一步优化实验条件，确定双水相萃取体系为PEG质量分数为30％、NaH2PO4质量分数为20％、体系pH为6，在此条件下Geotrichum sp．SYBC WU-3脂肪酶经硫酸铵沉淀和双水相萃取两步纯化的纯化倍数达到最大，较Geotrichum sp．SYBC WU-3脂肪酶粗酶纯化了22倍。Geotrichum sp．SYBC WU-3脂肪酶纯酶为低温碱性脂肪酶，最适反应温度为15oC，最适pH为9．5，相对分子质量为3．58×10^4。 |
| 关 键 词： | 双水相 酶学性质 Geotrichum sp． 脂肪酶 萃取 |
Aqueous two-phase extraction of lipase produced by Geotrichum sp. SYBC WU-3 and characterization of enzyme |
|
| WANG Lei,CAI Yu-jie,LIAO Xiang-ru,ZHANG Feng,ZHANG Da-bing.Aqueous two-phase extraction of lipase produced by Geotrichum sp. SYBC WU-3 and characterization of enzyme[J].Chinese Journal of Bioprocess Engineering,2009,7(2):57-62. | |
| Authors: | WANG Lei CAI Yu-jie LIAO Xiang-ru ZHANG Feng ZHANG Da-bing |
| Affiliation: | 1. School of Bioteehnology, Educational Ministry. Key Laboratory. of Industrial Biotechnology ,Jiangnan University, Wuxi 214122 ,China; 2. School of Biological and Chemical Engineering,Inner Mongolia University of Science and Technology,Baotou 014010,China; 3. Jiang Su Hanbon Science ＆ Technology Co. Ltd,Huai＇an 223001,China) |
| Abstract: | Aqueous two-phase system composed of PEG4000/NaH2PO4 was evaluated in the extraction of C, eotrichum sp. SYBC WU-3 lipase. Factors of molecular weight, concentration of PEG, concentration of Nail2 PO4, and pH on the lipase were investigated. The optimized conditions by orthogonal test design was as follows ： PEG concentration of 30% , NaH2PO4 concentration of 20% , pH 6. At these conditions, the activity of Geotrichum sp. SYBC WU-3 lipase was 22 times of the crude product. The lipase was a lowadapted alkaline lipase, the optimum temperature was 15 ℃, the optimum pH was 9.5, and the molecular weight was 3.58 × 10^4. |
| Keywords: | Geotrichum sp |
| 本文献已被 维普 万方数据 等数据库收录！ | |
