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酶法提取桑葚多糖的工艺研究
引用本文:刘晓露,刘胜利,郭立强,黄礼德,黄锁义.酶法提取桑葚多糖的工艺研究[J].中国野生植物资源,2012,31(1):30-32.
作者姓名:刘晓露  刘胜利  郭立强  黄礼德  黄锁义
作者单位:右江民族医学院,广西百色,533000
摘    要:利用纤维素酶从桑葚中提取桑葚多糖,通过单因素实验和L9(34)正交实验研究酶用量、酶解时间、酶解温度对桑葚多糖提取率的影响。实验结果表明:纤维素酶能够显著提高桑椹多糖的提取率,并且提取温度是最重要的影响因素,其次是酶解时间,酶用量在此实验范围内对测定结果的影响最小,提取的最佳工艺条件为:酶解温度45℃,酶解时间150 min,酶用量4.0 mL。

关 键 词:桑葚  多糖  纤维素酶  提取

Study on the Extraction of Mulberry Polysaccharide by Enzymatic Method
Liu Xiaolu , Liu Shengli , Guo Liqiang , Huang Lide , Huang Suoyi.Study on the Extraction of Mulberry Polysaccharide by Enzymatic Method[J].Chinese Wild Plant Resources,2012,31(1):30-32.
Authors:Liu Xiaolu  Liu Shengli  Guo Liqiang  Huang Lide  Huang Suoyi
Affiliation:*(Youjiang Medical University for Nationalities,Baise 533000,China)
Abstract:extraction of polysaccharide from mulberry fruit by cellulose enzyme was studied in this paper.Single factor and orthogonal design methods L9(34) were applied to analyze the effects of the factors such as the concentraction,time and temperature of the enzyme amount of mulberry polysaccharide.The results indicated that the temperature would significantly affected the extraction percent of mulberry polysaccharide.The optimum temperature was 45 ℃,the extracting time 150 min,and the enzyme amount 4.0 mL.
Keywords:mulberry fruit  polysaccharide  cellulose enzyme  extraction
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