Changes in the starvation response through covalent cell attachment |
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Authors: | Vladimir Jirkû |
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Affiliation: | (1) Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, 166 28, Prague 6, Czech Republic |
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Abstract: | Covalent attachment of Candida utilis cells, possibly simulating natural microbial immobilizations, stimulated stable and significant enhancement of extracellular production of alkaline protease, specifically induced by four different starvation conditions. The enzyme analysis confirmed the identity of the proteases released under all conditions of starvation and no parallel production of other proteolytic enzyme. The enhancement phenomenon as a uniform and stable effect of the whole cell immobilization is discussed in relation to the effect of multipoint, cell-solid surface contact, potentially bringing positive modulations of complex, cellular functions. |
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Keywords: | starvation stress protease production cell attachment spacer arm biofilm Candida utilis |
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