| 表达传染性法氏囊病病毒(IBDV)VP2基因的重组马立克氏病病毒的构建 |
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| 引用本文： | 周雪媚,李永清,张培君,王宏俊,佘锐萍,章振华,罗长保.表达传染性法氏囊病病毒(IBDV)VP2基因的重组马立克氏病病毒的构建[J].病毒学报,2005,21(6):474-477. |
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| 作者姓名： | 周雪媚 李永清 张培君 王宏俊 佘锐萍 章振华 罗长保 |
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| 作者单位： | 中国农业大学动物医学院,北京,100094;北京市农林科学院畜牧兽医研究所,北京,100089;北京市农林科学院畜牧兽医研究所,北京,100089;中国农业大学动物医学院,北京,100094;江西农业大学动物医学院,南昌,330045;广州出入境检疫检验局,广州,510623 |
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| 基金项目： | 国家863计划（No,2003AA24112002） |
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| 摘 要： | 传染性法氏囊病（Infectious bursal disease,IBD）是一种由鸡传染性法氏囊病病毒（Infectious bursal disease virus,IBDV）引起的危害3～12周龄青年鸡的急性、高度接触性传染病.IBDV属于双RNA病毒科的禽双RNA病毒属,其基因组由A和B两个节段组成.研究表明,IBDV主要的抗原性及致病性位点均位于A片段,其中VP2蛋白具有血清型特异性位点,并能诱导产生抗病毒的血清中和抗体,是该病毒的主要抗原.
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| 关 键 词： | 传染性法氏囊病病毒 VP2基因 重组马立克氏病毒 |
| 文章编号： | 1000-8721（2005）06-0474-04 |
| 收稿时间： | 2004-12-06 |
| 修稿时间： | 2004-12-062005-08-22 |
Construction of Recombinant Marek's Disease Virus Expressing Infectious Bursal Disease Virus VP2 Gene |
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| ZHOU Xue-mei,LI Yong-qing,ZHANG Pei-jun,WANG Hong-jun,SHE Rui-ping,ZHANG Zhen-hua,LUO Chang-bao.Construction of Recombinant Marek's Disease Virus Expressing Infectious Bursal Disease Virus VP2 Gene[J].Chinese Journal of Virology,2005,21(6):474-477. |
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| Authors: | ZHOU Xue-mei LI Yong-qing ZHANG Pei-jun WANG Hong-jun SHE Rui-ping ZHANG Zhen-hua LUO Chang-bao |
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| Affiliation: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China ; 2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China ; 3. College of Veterinary Medicine, Jiangxi Agricultural University, Nanchang 330045, China ; 4. Guangzhou Bureau of Entry-Exit Inspection and Quarantine, Guangzhou 510623, China |
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| Abstract: | In this study, a 1.35 kb DNA fragment encoding the VP2 protein of infectious bursal disease CJ801 isolate was obtained by PCR and the sequence was determined in T vector. The VP2 gene was cloned into the vector of pcDNATM4/His/LacZ, then the expression cassette including the VP2 gene of IBDV and LacZ gene of E. coli controlled by CMV promoter were inserted into US2 gene of MDV CVI988/Rispens to give a transfer vector of pUS2-VP2. The complex of pUS2-VP2 and DOTAP was transfected into MDV infected CEF. The recombinant MDV expressing LacZ gene were selected and purified on 96-well plate by blue plague. The complete VP2 gene inserted into MDV was detected by PCR. Western blot and imrnunostaining results demonstrated that VP2 protein of IBDV was expressed by recombinant MDV. |
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| Keywords: | infectious bursal disease virus VP2 gene recombinant Marek's disease virus |
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