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| 银杏愈伤组织培养及其黄酮类化合物的测定(简报) | |
| 引用本文： | 倪静静,黄学林,冈田芳明,黄家总.银杏愈伤组织培养及其黄酮类化合物的测定(简报)[J].热带亚热带植物学报,2001,9(2):163-166. |
| 作者姓名： | 倪静静 黄学林 冈田芳明 黄家总 |
| 作者单位： | 1. 中山大学生命科学学院, 2. 日本奈良县立山边立高等学校 3. 广东省农业科学院良种苗木中心, |
| 摘 要： | 采用银杏胚、胚乳及3个月苗龄的苗叶为外植体，在附加不同激素的培养基上诱导出愈伤组织，从愈伤组织中提取黄酮类化合物并测定其含量。结果表明，由胚诱导的愈伤组织中黄酮类化合物含量最高。 |
| 关 键 词： | 银杏 愈伤组织 黄酮类化合物 测定 |
| 文章编号： | 1005-3395(2001)02-0163-04 |
| 修稿时间： | 2000年4月10日 |
CALLUS CULTURE OF GINKGO AND THE CONTENT OF FLAVONOID COMPOUNDS IN THE CALLI |
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| NI Jing-jing,HUANG Xue-lin,YOSHIAKI Okad,HUANG Jia-zong.CALLUS CULTURE OF GINKGO AND THE CONTENT OF FLAVONOID COMPOUNDS IN THE CALLI[J].Journal of Tropical and Subtropical Botany,2001,9(2):163-166. | |
| Authors: | NI Jing-jing HUANG Xue-lin YOSHIAKI Okad HUANG Jia-zong |
| Abstract: | The embryos, endosperm and young leaves of 3-month-seedling of Ginkgo biloba L. were used as explants for callus induction experiment. Calli induced from embryos on MS medium supplemented with 3 mg L-1 NAA and 5 mg L-1 KT gave highest growth rate (100%), whereas calli induced from endosperms on MS with 3 mg L-1 NAA and 2 mg L-1 KT gave 42.3% growth rate. MS with 1 mg L-1 NAA and 3 mg L-1 BA was best for callus induction from leaves. The content of flavonoid compounds was highest in calli from embryos, and lowest in calli from endosperms. |
| Keywords: | Ginkgo biloba Callus induction Flavonoid compounds |
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