| MITE类转座子mPing在水稻不同亚种间的差异分析 |
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| 引用本文： | 张宁,阮亚男,王姗姗,刘洋,赵宸,王晶晶,王凯玺,王艳丽,王红艳.MITE类转座子mPing在水稻不同亚种间的差异分析[J].生物工程学报,2016,32(9):1264-1272. |
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| 作者姓名： | 张宁 阮亚男 王姗姗 刘洋 赵宸 王晶晶 王凯玺 王艳丽 王红艳 |
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| 作者单位： | 1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036；2 西藏自治区农牧科学院农业研究所 品种资源研究室，西藏 拉萨 850000,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,3 辽宁省水土保持研究所，辽宁 朝阳 122000,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036,1 辽宁大学 生命科学院 植物表观遗传与进化实验室，辽宁 沈阳 110036 |
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| 基金项目： | 国家自然科学基金 (No. 31100172)，辽宁省高等学校优秀人才支持计划 (No. LJQ2013003)，辽宁大学青年科研基金 (No. 2010LDQN04) 资助。 |
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| 摘 要： | mPing是水稻中第一个被鉴定出的有活性的MITE类转座子,为了探索mPing在水稻粳稻品种日本晴和籼稻品种93-11基因组中的分布差异,本研究首先运用Southern杂交的方法初步检测m Ping在两个亚种中拷贝数的差异,然后通过同源性探寻方法发现,m Ping在水稻亚种日本晴和93-11基因组中拷贝数分别为52和14,并且日本晴基因组中的m Ping均为m Ping-1,93-11中m Ping-1的拷贝数为3,m Ping-2的拷贝数为11。通过分析m Ping上下游5 kb侧翼序列发现m Ping在日本晴和93-11中分别与23和3个已知基因相关联。本研究为阐明以m Ping的分布多样性为主要原因的粳稻和籼稻之间的遗传差异提供初步理论基础。
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| 关 键 词： | 粳稻，籼稻，转座子，mPing，水稻 |
| 收稿时间： | 2015/12/25 0:00:00 |
Comparison of MITE transposons mPing in different rice subspecies |
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| Ning Zhang,Yanan Ruan,Shanshan Wang,Yang Liu,Chen Zhao,Jingjing Wang,Kaixi Wang,Yanli Wang and Hongyan Wang.Comparison of MITE transposons mPing in different rice subspecies[J].Chinese Journal of Biotechnology,2016,32(9):1264-1272. |
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| Authors: | Ning Zhang Yanan Ruan Shanshan Wang Yang Liu Chen Zhao Jingjing Wang Kaixi Wang Yanli Wang and Hongyan Wang |
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| Affiliation: | 1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China; 2 Germplasm Resources Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, Tibet, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China,3 Liaoning Institute of Soil and Water Conservation, Chaoyang 122000, Liaoning, China,1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China and 1 Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China |
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| Abstract: | The mPing family is the first active MITE TE family identified in rice genome. In order to compare the compositions and distributions of mPing family in the genomes of two rice subspecies japonica (cv. Nipponbare) and indica (cv. 93-11), we initially estimated the copy numbers of mPing family in those two subspecies using Southern blot and then confirmed the results by searching homologous copies in each reference genome using Blastn program, which turned out to have 52 and 14 mPing copies in corresponding reference genome, respectively. All mPing members in Nipponbare genome belong to mPing-1, while there are 3 mPing-1 and 11 mPing-2 copies in 93-11 genome. By further investigating the 5-kb flanking sequences of those mPing copies, it was found that 23 and 3 protein-coding genes in Nipponbare and 93-11 genome are residing adjacent to those mPing copies respectively. These results establish the preliminary theoretical foundation for further dissecting the genetic differences of japonica and indica rice in terms of the diversities and distributions of their component mPing. |
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| Keywords: | Japonica Indica transposon mPing rice |
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