Box-Behnken响应面法优化超声波辅助提取辣木叶多酚工艺研究

采用超声波辅助提取辣木Moringa oleifera叶多酚，通过单因素试验考察乙醇浓度、料液比、提取时间和提取次数对辣木叶多酚得率的影响，进一步利用四因素三水平Box-Behnken组合设计响应面试验对提取工艺参数进行优化。结果表明，超声波辅助提取辣木叶多酚的最佳工艺条件为乙醇浓度62%、料液比1∶30(W/V)、提取时间30 min、提取3次，在此条件下辣木叶多酚得率为27.32 mg·g-1，所得回归模型拟合情况良好。

Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology

The optimum process of ultrasound-assisted extraction of total polyphenos from Moringa oleifera leaves by response surface methodology was studied in the research. The experiment method was designed according to Box-Behnken central composite design with 4 factors and 3 levels on the basis of single-factor experiment of ethanol concentration, material-to-liquid ration, extraction time and extraction times. The relationship between influencing factors and response values was subsequently established. The results showed that the optimum extraction conditions were as follows: the extracting solvent was 62% ethanol, material to liquid ration was 1:30 (W/V), extracting time was 30 min, and extracting three times. Under these conditions, the maximal yield of total polyphenols was upto 27.32 mg·g-1, which was well matched with the predicted content.