超声波处理土样分离放线菌

【目的】探索超声波处理土壤悬液,增加稀有放线菌的类群。【方法】将西双版纳热带雨林的混合土样,制成土壤悬液,用超声波分别处理0-120s,用平板稀释法分离放线菌,得到纯菌落后测定其16S rRNA基因序列,进行系统发育分析,将分离菌株鉴定到属;用超声波处理已经鉴定到种且常见的10种链霉菌0-5min,后进行培养,测定其存活率。【结果】土壤悬液经超声波处理不同时间,放线菌的数量和种类逐渐增加。超声波处理已知链霉菌1-5min,对链霉菌的数量没有明显影响。【结论】用超声波处理土壤悬液40s,可以大大增加放线菌的出菌总数,明显增加稀有放线菌的种类,是一种经济且简便易行的方法。

Ultrasonic treatment of soil samples for actinomycete isolation

Abstract: Objective] We used ultrasonic to treat soil samples to isolate rare actinomycetes strains. Methods] We collected and suspended soil samples from mix tropical-rain-forest in Xishuangbanna. Farther, we treated the soil suspension with ultrasound for 10 to 120 s and used the plate dilution method to isolate actinomycetes. After got pure colonies, we sequenced their 16S rRNA gene sequences and calculated to put in phylogenetic analysis. The isolates were identified to the genus. We treated 10 kinds of common and identified Streptomyces with 1-5 min using ultrasonic, and then were cultured to measure their survival rate. Results] The soil suspensions treated with different times by the ultrasonic, actinomycetes gradual increased in the number and types. Ultrasonic treating of known Streptomyces with 0-5 min, there was no significant effect on the survival number of Streptomyces. Conclusion] The ultrasonic treating with soil suspensions for 40 s can significantly increase the total number of actinomycetes and the types of rare actinomycetes. Thereby, it is an economic and simple method to apparently increase the types of rare actinomycetes in the isolation.