| 台勾霉素生产菌指孢囊菌NRRL 18085遗传操作体系的建立 |
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| 引用本文： | 肖毅,李苏梅,马亮,张改云,鞠建华,张长生.台勾霉素生产菌指孢囊菌NRRL 18085遗传操作体系的建立[J].微生物学报,2010,50(8):1014-1022. |
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| 作者姓名： | 肖毅 李苏梅 马亮 张改云 鞠建华 张长生 |
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| 作者单位： | 海洋生物资源可持续利用重点实验室,中国科学院海洋微生物研究中心,广东省海洋药物重点实验室,中国科学院南海海洋研究所,广州,510301 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目(KZCX2-YW-216)，国家自然科学基金青年科学基金（30900035），中国博士后科学基金（20090460836），中国科学院百人计划项目（08SL111002） |
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| 摘 要： | 【目的】建立新型大环内酯类抗生素台勾霉素的生产菌指孢囊菌Dactylosporangium aurantiacum NRRL18085的遗传操作体系,实现台勾霉素相关生物合成基因的敲除突变。【方法】以整合型质粒pSET152为载体,建立了外源DNA通过接合转移进入指孢囊菌NRRL18085的操作方法和培养条件,利用PCR-targeting系统在体外构建了一个台勾霉素卤化酶基因敲除的cosmid质粒,通过接合转移转入到指孢囊菌NRRL18085野生菌中。【结果】获得了台勾霉素卤化酶基因敲除的指孢囊菌NRRL18085的双交换突变株,该突变株失去了产生台勾霉素的能力。【结论】成功建立和优化了指孢囊菌NRRL18085菌株的遗传操作体系,使得在体内分析和鉴定台勾霉素生物合成基因的功能成为可能,同时也为建立其他类似放线菌的遗传操作体系提供了参考。
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| 关 键 词： | 关键词: 指孢囊菌 遗传体系 台勾霉素 生物合成 卤化酶 |
| 收稿时间： | 2010/1/29 0:00:00 |
| 修稿时间： | 2010/4/30 0:00:00 |
Genetic modification system for tiacumicin producer Dactylosporangium aurantiacum NRRL 18085 |
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| Yi Xiao,Sumei Li,Liang M,Gaiyun Zhang,Jianhua Ju'' and Changsheng Zhang.Genetic modification system for tiacumicin producer Dactylosporangium aurantiacum NRRL 18085[J].Acta Microbiologica Sinica,2010,50(8):1014-1022. |
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| Authors: | Yi Xiao Sumei Li Liang M Gaiyun Zhang Jianhua Ju' and Changsheng Zhang |
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| Affiliation: | Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Research Center of Marine Microbes, Key Laboratory of Marine Bio-resource Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China |
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| Abstract: | |
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| Keywords: | Keywords: Dactylosporangium Genetic manipulation system Tiacumicin Biosynthesis Halogenase |
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