| 赤霉菌超氧化物歧化酶的纯化及部分理化性质 |
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| 引用本文： | 口如琴,白玉明,袁静明,褚西宁.赤霉菌超氧化物歧化酶的纯化及部分理化性质[J].微生物学报,1997,37(2):115-118. |
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| 作者姓名： | 口如琴 白玉明 袁静明 褚西宁 |
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| 作者单位： | 山西大学分子科学研究所；山西大学分子科学研究所 太原 030006现通讯地址北京2732信箱 100080 |
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| 摘 要： | 采用加热、Sephadex G—100凝胶过滤及DEAE-Sephadex A-50柱层析的方法,提纯了赤霉菌的超氧化物歧化酶(SOD),纯酶比活力为2640U/mg蛋白,最大紫外吸收峰为276nm,为Mn-SOD,由二个亚基组成,亚基分子量为14.5kD。此外还报道了该酶的氨基酸组成。
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| 关 键 词： | 赤霉菌 超氧化物歧化酶 纯化和性质 |
PURIFICATION AND SOME PROPERTIES OF SUPEROXIDE DISMUTASE FROM FUSARIUM MONILIFORM |
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| Kou Ruqin Bai Yuming Yuan Jingming Chu Xining.PURIFICATION AND SOME PROPERTIES OF SUPEROXIDE DISMUTASE FROM FUSARIUM MONILIFORM[J].Acta Microbiologica Sinica,1997,37(2):115-118. |
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| Authors: | Kou Ruqin Bai Yuming Yuan Jingming Chu Xining |
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| Abstract: | Superoxide dismutase from Fusarium moniliform was purified by the steps including heating, ammonium sulfate fractionation, Sephedax G-100 gel filtration and DEAE - Sephadex A-50 chromatography. The results showed that the enzyme was a Mn-SOD with the specific activity of 2640 U /mg and had two homogenous subunits whose molecular mass were 14.5 kD. The wave length of max. absorbing peak in ultraviolet spectrum was 276 nm which was not similar with other resource of SOD. The composition of amino acid was also analyzed. |
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| Keywords: | Fusarium Moniliform Superoxide dismutase Purification and properties |
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