新疆维吾尔族、哈萨克族2型糖尿患者群肠道菌群中直肠真杆菌与多形拟杆菌的定量研究

目的分析新疆维吾尔族、哈萨克族正常糖耐量人群和2型糖尿患者群肠道菌群中直肠真杆菌与多形拟杆菌的量变差异,探讨肠道菌群与2型糖尿病（T2DM）的相关性。方法采用16S rDNA实时荧光定量RT-PCR技术相对定量法。结果分别比较4组人群肠道菌群中直肠真杆菌与多形拟杆菌数量的对数值,与维吾尔族正常组比较,该民族T2DM组中直肠真杆菌的量差异有统计学意义（P=0.0125）,与哈萨克族正常组比较,该民族T2DM组中直肠真杆菌的量差异有统计学意义（P=0.0261）,两民族正常组、T2DM组之间直肠真杆菌的量差异均未见统计学意义;与维吾尔族正常组比较,该民族T2DM组中多形拟杆菌的量差异有统计学意义（P=0.0003）,哈萨克族T2DM组与正常组中多形拟杆菌的量差异有统计学意义（P=0.0055）,两民族正常组之间多形拟杆菌的量差异有统计学意义（P=0.0154）,两民族T2DM组之间多形拟杆菌的量差异未见统计学意义。结论直肠真杆菌与多形拟杆菌数量的变化,与新疆维吾尔族、哈萨克族2型糖尿病的发生（可能）相关,需要进一步深入探讨。

The quantitative research of Eubacterium rectale and Bacteroides multiforme in intestinal microflra of Normal Glucose Tolerance and Type 2 diabetes mellitus in Uygur and Kazak population in Xinjiang

Objective To analyze the quantitative differences of Eubacterium rectale and Bacteroides multiforme in intestinal microflra of Normal glucose tolerance and Type 2 diabetes mellitus（T2DM） in Uygur and Kazak population,and explore the correlation between intestinal microflora and T2DM.Method Relative quantification of 16S rDNA Real Time PCR was used.Result Compared NGT and T2DM in Uygur and Kazak population,we found the quantity of Eubacterium rectale had a statistically significant difference respectively（P=0.0125,P=0.0261）.There was no different band between the quantity of Eubacterium rectale in T2DM and NGT of these two national.Compared NGT and T2DM in Uygur and Kazak population,we found the quantity of Bacteroides multiforme had a statistically significant difference separately（P=0.0003,P=0.0055）,and the NGT of the two national had a statistically significant difference（P=0.0154）.But there was no different between the quantity of Bacteroides multiforme in T2DM of the two national.Conclusion The changing of target bacteria may be related to the onset of Type 2 diabetes mellitus in Uygur and Kazak.