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| 健康儿童与轮状病毒感染儿童肠道菌群结构的比较研究 | |
| 引用本文： | 张美玲,杜惠敏,郭美蕻,陈俊仪,庞小燕,赵立平.健康儿童与轮状病毒感染儿童肠道菌群结构的比较研究[J].中国微生态学杂志,2006,18(3):164-166. |
| 作者姓名： | 张美玲 杜惠敏 郭美蕻 陈俊仪 庞小燕 赵立平 |
| 作者单位： | 1. 上海交通大学生命科学技术学院,微生物分子生态学与生态基因组学实验室,上海,200240 2. 上海交通大学附属新华医院,上海,200092 3. 上海交通大学附属幼儿园,上海,200240 |
| 摘 要： | 目的比较分析轮状病毒感染个体与健康个体肠道菌群结构的差异。方法采集11例轮状病毒感染个体及6例健康个体的粪便样品，提取粪便样品中细菌的混合DNA，先通过ERIC-PCR结合分子杂交的技术分析两组个体之间肠道微生物组成的相似性；再扩增粪便样品中菌群的16SrRNA基因，利用PCR—TGGE技术分析肠道菌群的组成情况。结果轮状病毒感染个体与健康个体相比，肠道菌群中GC含量较低的菌明显减少，同时肠道菌群有宿主专一性。结论轮状病毒感染会导致儿童肠道内菌群结构失调。 |
| 关 键 词： | 轮状病毒 肠道菌群 |
| 文章编号： | 1005-376X（2006）03-0164-03 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2005年12月12 |
Comparison of microbial community structure in healthy and rotavirus infected children |
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| ZHANG Mei-ling,DU Hui-ming,GUO Mei-hong,CHEN Jun-yi,PANG Xiao-yan,ZHAO Li-ping.Comparison of microbial community structure in healthy and rotavirus infected children[J].Chinese Journal of Microecology,2006,18(3):164-166. | |
| Authors: | ZHANG Mei-ling DU Hui-ming GUO Mei-hong CHEN Jun-yi PANG Xiao-yan ZHAO Li-ping |
| Affiliation: | 1. Lab of Molecular Microbial Ecology, Shanghai Jiaotong University, Shanghai 200240, China;2. Shanghai Jiao Tong University Affiliated Xinhua Hospital, Shanghai 200092, China ; 3. Shanghai Jiao Tong University Affiliated Kindergarfen , Shanghai 200240, China |
| Abstract: | Objective To compare the composition of the microbial comn,unity in healthy children and rotavirus infected children. Methods Fecal samples from 11 rotavirus infected children and 6 healthy children were collected. The total genomic DNA was extracted from each sample. Molecular hybridization was performed with a mixed probe from the labeled ERIC-PCR product of one sample, and PCR-TGGE analysis of amplified 16S rDNA V3 regions was carried out. Results Low-GC bacteria were less in rotavirus infected children thann healthy ones. Intestinal microflora was host-specific. Conclusion Rotavirus infection could cause structural shifts of the gut microflora. |
| Keywords: | ERIC-PCR PCR-TGGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
