痰标本涂片检查与培养结果分析

目的：探讨痰标本涂片检查在细菌培养以及临床治疗中的意义。方法：送检标本首先直接涂片革兰染色镜检，同时将合格痰标本划分为以阴性杆菌、阳性球菌、霉菌为主和普通标本四大类。再对合格痰标本行细菌学培养及药物敏感测定。结果：314份痰标本合格率为62．4％(196／314)。196份合格痰标本共检出145株病原菌，培养阳性率74．0％。涂片以阴性杆菌、阳性球菌、霉菌为主的标本与培养结果符合率分别为93．1％、68．4％和41．2％。结论：痰培养结果的准确性受痰标本是否合格、病原菌的生长速度以及是否使用过大剂量抗生素等因素直接影响。临床医生必须依据临床症状及涂片结果判断作出何种为真正的病原菌。

ANALYSIS THE RESULT FROM SMEAR AND BACTERIAL CULTURE

Objective: To evaluate the values of sputum smear for bacterial culture and therapeutic.Methods:Sputum specimens were directly examined under microscope by Gram staining.Qualified sputum specimens were cultured for bacterial isolation,and the antimicrobial activity tests.Partition qualified sputum specimens into Gram positvie cocci,Gram negative rods,fungus and natural specimens.Results: The qualify rate for three hundred and fourteen sputum specimens was 62.4%(196/314).One hundred and forty-five patogenic agents were isolated from 196 qualified sputum specimens,the isolating rate was 74.0%. Smear and culture bacteria were Gram negative rods,Gram positive cocci and fungus the isolating rates being 93.1%,68.4% and 41.2%.Except that eight shares were Gram positive cocci to culture also.Ten shares of sputum specimens were examined fungus,culture weren't it,six shares were examined fungus when it's overtime.Conclusions: The result in bacterial culture was correlated with qualify of sputum specimens,growth speed of pathogenicbacteria, treatment received previously,etc.So clincian must judging the pathogenicbacteria by the result form smear and clinical symptom.