| 两个水稻胚乳启动子的克隆及表达分析 |
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| 引用本文： | 崔永兰,钟晓丽,张永明,杨仲南.两个水稻胚乳启动子的克隆及表达分析[J].中国生物工程杂志,2008,28(8):57-61. |
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| 作者姓名： | 崔永兰 钟晓丽 张永明 杨仲南 |
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| 作者单位： | 上海师范大学生命与环境科学学院 上海师范大学生命与环境科学学院 上海师范大学生命与环境科学学院 |
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| 基金项目： | 上海市高校优秀青年教师后备人选科研项目，上海市教委资助项目 |
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| 摘 要： | 启动子的克隆对基因表达及基因工程研究有重要意义。根据数据库中EST丰度，从水稻中克隆了两个预测在水稻胚乳中高效表达的启动子Os772和Os359，并将启动子片段与GUS报告基因融合，构建了重组表达载体。通过农杆菌介导方法将其导入水稻愈伤组织细胞。转基因水稻经GUS组织化学分析显示，Os772和Os359能启动GUS基因在水稻胚乳中表达但不能在根、茎、叶和花中表达。该结果表明Os772和Os359为两个水稻胚乳特异性启动子。
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| 关 键 词： | 水稻 胚乳启动子 GUS报告基因 |
| 收稿时间： | 2008-03-04 |
| 修稿时间： | 2008-03-28 |
Cloning and Expression Analysis of two Endosperm Promoters in Rice |
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| CUI Yong-lan,ZHONG Xiao-li,ZHANG Yong-ming,YANG Zhong-nan.Cloning and Expression Analysis of two Endosperm Promoters in Rice[J].China Biotechnology,2008,28(8):57-61. |
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| Authors: | CUI Yong-lan ZHONG Xiao-li ZHANG Yong-ming YANG Zhong-nan |
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| Abstract: | The cloning of promoter is important for studying the genetic engineering and the regulation of gene expression in plants. In this paper, we cloned two promoters Os772 and Os359, which are predicted to be highly expressed in the endosperm of rice from the EST database. After construction of the Os772::GUS and Os359::GUS expression vectors, they were transformed into rice. X-Gluc staining of transgenic plants showed that Os772 and Os359 can promote GUS gene expression in matured endosperm but not in root, stem, leaf and flower. This result indicates Os772 and Os359 are two rice endosperm-specific promoters. |
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| Keywords: | Rice (Oryza sativa L) Endosperm promoter GUS reporter |
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