| RAPD技术及其在动物遗传育种中的应用 |
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| 引用本文： | 张丕燕,谢庄,刘红林,陈杰.RAPD技术及其在动物遗传育种中的应用[J].中国生物工程杂志,2000,20(4):52-54. |
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| 作者姓名： | 张丕燕 谢庄 刘红林 陈杰 |
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| 作者单位： | 南京农业大学动物科技学院 南京 210095 |
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| 摘 要： | RAPD技术是在PCR基础上发展起来的一种DNA多态性检测技术,已广泛应用于基因组研究的各个领域。本文概述了RAPD反应的原理、特点,总结了其在遗传多样性检测、亲缘关系鉴定、遗传连锁分析和数量性状的辅助标记选择等方面的应用,并肯定了RAPD在动物遗传育种领域的应用前景。
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| 关 键 词： | RAPD 遗传多样性 遗传连锁分析 亲缘关系 辅助标记选择 |
RAPD Analysis Technique and It's Application to The Studies on The Animal Genetics and Breeding |
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| Zhang Piyan,Xie Zhuang,Liu Honglin,Chen Jie.RAPD Analysis Technique and It's Application to The Studies on The Animal Genetics and Breeding[J].China Biotechnology,2000,20(4):52-54. |
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| Authors: | Zhang Piyan Xie Zhuang Liu Honglin Chen Jie |
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| Abstract: | Random amplified polymorphic DNA(RAPD),a polymerase chain reaction based technique testing DNA polymorphisms,has been widely used in genome research.In the field of animal genetics and breeding,RAPD technique provides a powerful tool for detection of gene diversity of populations and analysis of genetic relationship.It also can be used for analysis of linkage between RAPD markers and quantitative trait loci(QTL) and Markers Assisted Selection (MAS).Theory and features of RAPD were also discussed in this paper.According to the authors,RAPD technique has good perspective. |
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| Keywords: | RAPD Gene Diversity Genetic Relationship Linkage Analysis MAS |
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