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兔出血症病毒主要结构多肽的氨基酸分析
引用本文:王恒安,杜念兴.兔出血症病毒主要结构多肽的氨基酸分析[J].Virologica Sinica,1995,10(2):180-184.
作者姓名:王恒安  杜念兴
作者单位:南京农业大学,上海动物保健公司申宝分公司
摘    要:兔出血症病毒主要结构多肽的氨基酸分析王恒安,杜念兴,徐为燕(南京农业大学,南京210095)关键词兔出血症病毒,结构多肽,氨基酸分析有关兔出血症病毒(RHDV)结构多肽的报道很多,有认为只有1条,有报道4条的,也有报道多达6条的。但其主要结构多肽为分...

关 键 词:兔出血症病毒  结构多肽  氨基酸分析

Amino Acid Analysis of the Major Structural Polypeptides of Rabbit Haemorrhagic Disease Virus
Wang Hengan,Du Nianxing,Xu Weiyan.Amino Acid Analysis of the Major Structural Polypeptides of Rabbit Haemorrhagic Disease Virus[J].中国病毒学(英文版),1995,10(2):180-184.
Authors:Wang Hengan  Du Nianxing  Xu Weiyan
Abstract:abbit haemorrhagic disease virus(RHDV)was purified from infected liver by freezing and thawing,chloroform extraction,PEG 6 000 precipitation and Sepharose-4B chromatography.Six polypeptides ,with the molecular weights of 64.5kD,61.4kD, 57.3kD,54.0kD.29.2kD and 28.1kD weredetermined by immuno blotting to be viral structural polypeptides.The major strucrural polypeptide(64.5kD),purified electrophoretically was analysed by hydrochloricacid lysis and fourteen kinds ofamino acids were found,of which,the base,neutral and acidic amino acids accounted for 75.14%,21.16% and 3.60%,respectively.
Keywords:Rabbit Haemorrhagic Disasee Virus  Structural polypoptide  Analysis of amino acids
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