| Caveolin-1真核表达载体的构建及其Hepa1-6细胞中的表达 |
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| 引用本文： | 汪淑晶,汪晓敏,师伟,张嘉宁.Caveolin-1真核表达载体的构建及其Hepa1-6细胞中的表达[J].生物学杂志,2011,28(6). |
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| 作者姓名： | 汪淑晶 汪晓敏 师伟 张嘉宁 |
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| 作者单位： | 大连医科大学生物化学与分子生物学教研室糖生物学研究所,大连,116044 |
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| 摘 要： | Caveolin-1在不同肿瘤中发挥作用不同,既发挥抑癌基因样作用又发挥癌基因样作用.旨在分析caveolin-1 在小鼠肝癌细胞系中的表达情况及建立稳定表达外源caveolin-1的Hepa1-6细胞.利用RT-PCR和Western-blot方法检测caveolin-1在小鼠肝癌H22、Hea-F和Hepa1-6细胞中的表达;通过分子克隆构建小鼠caveolin-1 cDNA真核表达栽体,利用脂质体转染等方法建立稳定表达外源caveolin-1的Hepa1-6细胞株;通过RT-PCR、Western-blot、免疫细胞化学等方法鉴定其稳定表达细胞株.结果显示,caveolin-1在Hepa1-6细胞中表达呈阴性,在H22和Hca-F 中高表达;成功获得小鼠caveolin-1 cDNA真核表达载体pEGFP-N2/Cav-1,筛选并鉴定出高表达外源caveolin-1的Hepa1-6稳定细胞株C1和C4,为进一步分析caveolin-1在肝癌中所发挥的作用奠定了一定的研究基础.
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| 关 键 词： | caveolin-1 分子克隆 转染 肝癌 |
Construction and expression of caveolin-1 expression vector in hepa1-6 cells |
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| WANG Shu-jing,WANG Xiao-min,SHI Wei,ZHANG Jia-ning.Construction and expression of caveolin-1 expression vector in hepa1-6 cells[J].Journal of Biology,2011,28(6). |
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| Authors: | WANG Shu-jing WANG Xiao-min SHI Wei ZHANG Jia-ning |
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| Affiliation: | WANG Shu-jing,WANG Xiao-min,SHI Wei,ZHANG Jia-ning(Department of Biochemistry and Molecular Biology,Institute of Glycobiology,Dalian Medical University,Dalian 116044,China) |
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| Abstract: | Caveolin-1 plays different roles in different kinds of tumors.Caveoiln-1 may act as tumor-suppressor,as well as tumor-promoter.This study aims to analyze the expression of caveolin-1 in mouse hepatoma cell lines and construct stable hepa1-6 cells expressing exogenous caveolin-1.The expression of caveolin-1 in mouse hepatoma cell lines(H22,Hca-F and Hepa1-6)was investigated by RT-PCR and Western-blot assays;mouse caveolin-1 cDNA expression vector was constructed by molecular clone,and stable hepa1-6 cells tr... |
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| Keywords: | caveolin-1 molecular clone transfection hepatoma |
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