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喹啉废水反硝化反应器中优势菌的代谢功能分析
引用本文:仲汇慧,张晓君,岳思青,袁飞,王凌华,赵立平.喹啉废水反硝化反应器中优势菌的代谢功能分析[J].微生物学通报,2010,37(5):0645-0650.
作者姓名:仲汇慧  张晓君  岳思青  袁飞  王凌华  赵立平
作者单位:上海交通大学生命科学技术学院教育部微生物代谢重点实验室,上海,200240
基金项目:国家自然科学基金项目(No. 20677041); 国家863计划项目(No. 2007AA021301); 上海市重点学科建设项目(No. B203)
摘    要:采用纯培养技术对一个降解喹啉的反硝化反应器筛选到的26株优势菌株进行反硝化能力以及好氧降解喹啉能力研究,其中的反硝化菌还测定了反硝化条件下的喹啉降解能力。结果发现Bacillus、Staphylococcus、Pseudomonas、Brucella、Delftia等5个属的10株菌具有反硝化能力,Rhodococcus属的9株细菌能够好氧降解喹啉,揭示了反硝化喹啉降解反应器中主要细菌类型的代谢特性,发现在缺氧反硝化反应器中存在多样的代谢类型的细菌。

关 键 词:喹啉    反硝化    降解率    优势细菌    群落

Analysis for Metabolic Activity of Predominant Bacterial Isolates in a Lab-scale Denitrifying Bioreactor for Quinoline-degradation
ZHONG Hui-Hui,ZHANG Xiao-Jun,YUE Si-Qing,YUAN Fei,WANG Ling-Hua and ZHAO Li-Ping.Analysis for Metabolic Activity of Predominant Bacterial Isolates in a Lab-scale Denitrifying Bioreactor for Quinoline-degradation[J].Microbiology,2010,37(5):0645-0650.
Authors:ZHONG Hui-Hui  ZHANG Xiao-Jun  YUE Si-Qing  YUAN Fei  WANG Ling-Hua and ZHAO Li-Ping
Affiliation:Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract:Using the pure culture technology, we analyzed 26 predominant bacterial strains isolated from a denitrifying quinoline-degrading bioreactor. The capacity of denitrification and aerobic degradation for quinoline of these strains was determined. Then the ability of denitrifying degradation of quinoline by selected denitrifying strains was measured. The results showed that 10 strains of isolates, which belong to Bacillus, Staphylococcus, Pseudomonas, Brucella and Delftia, were denitrifying bacteria. Whereas, 9 strains of isolates belonging to Rhodococcus had the ability of quinoline degradation under aerobic condition. This work described the metabolism function of the major predominant bacteria of microbial community in a quinoline degrading bioreactor. Diverse bacteria, in terms of metabolism, existed in the anoxic denitrifying bioreactor.
Keywords:Quinoline  Denitrification  Degrading efficiency  Predominant bacteria  Community
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