用5种同工酶分析云南芋种质资源的遗传多样性

用5种同工酶(酯酶、过氧化物酶、超氧化物歧化酶、多酚氧化酶、细胞色素氧化酶)分析云南48份芋材料遗传多样性,并将5种同工酶的酶带检测结果进行聚类分析.结果表明:48份材料的5种同工酶的酶带数分别在11～19条之间,除超氧化物歧化酶仅有3种酶谱类型外,其余4种同工酶的酶谱数均超过检测试材的半数.将聚类结果与试材的植物学性状相比较,发现以来源、用途、生态位和叶柄、叶心颜色等能对聚类结果进行初步分类,并给予合理解释.因此从试材的植物学性状观察、野生和半野生种的存在以及蛋白质表达水平的同工酶分析结果来看,云南省的芋种质资源呈现丰富的遗传多样性.这为芋遗传变异的进一步深入研究以及对正面临巨大威胁的云南芋资源进行原生境保存的可能性提供了依据.

Analysis of Genetic Diversity of Germplasm Resources in Taro Using Five Isozymes

Through the analysis of genetic diversity for 48 taro materials of Yunnan province using five isozymes of esterase, peroxidase, superoxidase, polyphenol oxidase, cytochrome oxidase and cluster analysis for their bands determined, the results showed that band number of five isozymes was between 11 and 19, the number of zymograms was all more than half number of materials determined except that SOD only showed three zymograms. Compared with their morphological characters, cluster result could be explained rationally by the source, use, zoological location, petiole color or the color of leaf center and preliminary classification could be made. Therefore, taro germplasm resources of Yunnan province presented great genetic diversity through observation of morphological characters, existence of wild, semi-wild varieties and isozyme analysis at protein expression level. The results could be used for further researches of taro genetic variation and in situ preservation for taro genetic resources in Yunnan province, which are facing great threat now.