SIK1作为miR-93新的靶基因抑制前列腺癌细胞增殖、侵袭和迁移

该文探讨了SIK1作为miR-93新的靶基因对前列腺癌细胞增殖、侵袭和迁移的抑制作用。采用重组质粒pcDNA3.1-SIK1上调前列腺癌细胞中SIK1的表达后,利用CCK8和克隆形成实验检测细胞增殖;利用细胞划痕和Transwell实验检测细胞侵袭和迁移;利用Western blot检测E-cadherin和Vimentin的蛋白表达。采用生物信息学方法预测靶向SIK1 mRNA的3’UTR的miRNAs并进行筛选;双荧光素酶报告实验和Western blot验证miR-93靶向调控SIK1。结果显示,上调SIK1的表达能抑制前列腺癌细胞的增殖、侵袭和迁移,并增加E-cadherin和减少Vimentin蛋白表达;miR-93能够靶向负调控SIK1。总之,SIK1可作为miR-93一个新的靶基因抑制前列腺癌细胞增殖、侵袭和迁移。

SIK1,As a New Target Gene of miR-93,Inhibits the Proliferation,Invasion and Migration of Prostate Cancer Cells

This paper investigated the inhibitory effect of SIK1,as a new target gene of miR-93,on the proliferation,invasion and migration of prostate cancer cells.After the expression of SIK1 in prostate cancer cells was up-regulated by recombinant plasmid pcDNA3.1-SIK1,CCK8 and clone formation assay were used to detect cell proliferation;cell invasion and migration was detected by cell scratches and Transwell assay;the protein expression levels of E-cadherin and Vimentin were detected by Western blot.Luciferase reporting experiment and Western blot verified the targeted regulation of SIK1 by miR-93.The results showed that up-regulated SIK1 could inhibit the proliferation,invasion and migration of prostate cancer cells,as well as increase E-cadherin protein expression and decrease Vimentin protein expression;miR-93 could negatively regulate SIK1.In conclusion,SIK1 can act as a new target gene of miR-93 to inhibit the proliferation,invasion and migration of prostate cancer cells.