黄芪注射液对2型糖尿病动物模型KKAy小鼠脑微血管病变的影响

目的：观察黄芪注射液对2型糖尿病动物模型KKAy小鼠脑微血管病变的影响，探讨黄芪注射液对糖尿病脑血管病变的保护作用。方法：饲养至14周龄的雄性KKAy小鼠随机分成模型组和黄芪注射液治疗组（每日腹腔给药，剂量为3mL／kg），同龄雄性C57BL／6J小鼠作为对照组。血糖仪测量20、24、28周龄时各组小鼠的空腹血糖水平。28周龄时处死各组小鼠，放射免疫法检测血清6-酮-前列腺素-F1α（6-Keto-PGF1α）和血栓素B2（TXB2）的含量。透射电子显微镜观察脑组织超微结构变化。结果：模型组KKAy小鼠从20周龄开始血糖水平明显高于正常组小鼠（P〈0．01）；黄芪治疗组小鼠从20周龄开始血糖水平明显高于正常组小鼠（P〈0．01），但低于模型组小鼠（P〈0．05或P〈0．01）。模型组小鼠血清6-Keto—PGF1α水平较正常组降低（P〈0．01），TXB2含量增高（P〈0．01）；与模型组相比，黄芪注射液治疗组小鼠6-Keto—PGF1α水平升高（P〈O．01），TXB：含量下降（P〈0．01）。透射电镜显示模型组小鼠神经细胞胞核染色质疏松，线粒体肿胀，粗面内质网缩小，核糖体减少；治疗组小鼠以上病变明显改善。结论：黄芪注射液可以有效改善2型糖尿病动物模型KKAv小鼠脑微血管病变，保护神经细胞结构。

Effect of Astragalus Injection on Type 2 Diabetes Animal Model of KKAy Mice Brain Microvascular Lesions

ABSTRACT Objective： To observe the cerebral microvascular pathological changes in KKAy mice with type 2 diabetes animal model, and investigate the mechanism of astragalus injection on diabetic cerebrovascular disease. Methods： The 14-week-old male KKAy mice were randomly divided into model group and astragalus injection treatment group, while the same age male C57BL/6J mice were selected as normal group, the fasting blood glucose of mice at 20,24 and 28 weeks of age in each group were measured. The animals were sacrificed at 28 weeks of age, and using radioimmunoassay to detect the content of serum 6-keto-prostaglandin-F1 alpha （6-Ke- to-PGF1α） and thromboxane B2（TXB2）. We used transmission electron microscope（TEM） to check the changes of ultrastructure in brain tissue. Results： When KKAy mice was 20 weeks old, the blood glucose levels of KKAy mice in the model group were significantly higher than the mice of normal group （P〈0.01）; astragalus injection treated mice from 20 weeks of age, blood glucose was significantly higher than the normal group mice （P〈0.01）, but lower than the model group mice （P〈0.05 or P〈0.01）. The serum 6-Keto-PGF1α levels of mice in model group compared with normal group decreased （P〈0.01）, TXB2 increased （P〈0.01）; and compared with the model group, the levels of 6-Keto-PGF1α elevated in astragalus injection treatment group （P〈0.01）, and TXB2 content decreased （P〈0.01）. Transmission electron microscopy revealed in the model mice of nerve cell nuclear chromatin loosing, swelling of mitochondria, rough endoplasmic reticulum narrow, reduction of ribosome; but the lesions in the mice treated with astragalus injection were markedly improved. Conclusion： Astragalus injection can effectively improve cerebral microvascular pathological changes in the type 2 diabetic KKAy mice, and protect the neurocyte structure. Key words： Type 2 diabetes; KKAy mice; Astragalus injection; Cerebral microvessels; Ultrastructure