胰岛素样生长因子-1受体（IGF—IR）在乳腺癌组织中的表达及其临床意义初探

目的：检测分析胰岛素样生长因子-1受体（IGF-IR）在乳腺癌组织中的表达状况及其临床意义。方法：应用半运用半定量RT-PCR方法分析84例乳腺癌和癌旁正常乳腺组织中IGF—IR基因mRNA的表达水平，并分析其表达与患者临床病理特征及预后之间的关系。结果：乳腺癌组织中IGF-IR基因mRNA表达水平显著高于癌旁乳腺组织，二者具有统计学差别（P〈0．001）。乳腺癌组织中IGF-IR基因mRNA表达水平与肿瘤组织分化程度及乳腺癌患者的TNM分期和淋巴结转移情况显著相关（P值分别是0．005，0．025和0．041）。另外，高表达IGF-IR的乳腺癌患者的五年总体生存率（38．3％）显著高于低表达IGF-1R的患者（49．7％；P=0．009）。多因素COX模型分析结果表明：IGF-IR基因mRNA表达水平是乳腺癌患者的一个独立预后分子（HR=2．78，95％CI：1．94-3．94，P=0．041）。结论：IGF-IR基因表达水平上调在乳腺癌发展过程中起着重要的作用。IGF-IR基因mRNA表达水平有望成为临床乳腺癌患者预后判断的一个重要分子标志物。

Expression of Insulin-Like Growth Factor-1 Receptor mRNA in Human Breast Cancer Tissues and Its Clinical Significance

Objective： To investigate the expression of IGF-1R mRNA in human breast cancer tissues and its prognostic roles. Methods： Semi-quantitative RT-PCR assay was employed to detect the expression of IGF-IR mRNA in 82 breast cancer and correspond- ing normal breast epithelial tissues. Additionally, the correlation of IGF-1R mRNA expression with clinicopathological factors of breast cancer patients was investigated and its correlation with prognosis of breast cancer patients was also investigated. Results： The level of IGF-1R mRNA expression was significantly higher than that in corresponding normal breast epithelial tissues （P〈0.001）. High level of IGF-1R mRNA expression was significantly correlated with tumor differentiation, TNM stage and lymph node metastasis of breast cancer patients （P=0.005, 0.025 and 0.041, respectively）. The 5-year overall survival rate of breast cancer patients with high IGF-IR mRNA （38.3%） was significantly shorter than that in those patients with low IGF-1R mRNA （49.7%; P=0.009）. Multivariate analysis of COX model showed that IGF-1R mRNA expression was an independent prognostic factor for breast cancer patients （HR=2.78,95%CI： 1.94-3.94, P=0.041）. Conclusion： The upregulation of IGF-1R mRNA might play important roles in progression of breast cancer and the status of IGF-1R mRNA expression has potential of being an important biomarker for breast cancer patients.