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| 保存介质和温度对西伯利亚鲟卵子短期保存的影响 | |
| 引用本文： | 张涛,颜世伟,庄平,章龙珍,田美平,王斌,张大海,姚志峰.保存介质和温度对西伯利亚鲟卵子短期保存的影响[J].应用生态学报,2010,21(1):227-231. |
| 作者姓名： | 张涛 颜世伟 庄平 章龙珍 田美平 王斌 张大海 姚志峰 |
| 作者单位： | 1. 中国水产科学研究院东海水产研究所农业部海洋与河口渔业资源及生态重点开放实验室,上海,200090 2. 大连水产学院生命科学与技术学院,辽宁大连,116023 3. 中国水产科学研究院东海水产研究所农业部海洋与河口渔业资源及生态重点开放实验室,上海200090;大连水产学院生命科学与技术学院,辽宁大连116023;上海海洋大学水产与生命学院,上海201306 4. 上海海洋大学水产与生命学院,上海,201306 5. 杭州千岛湖鲟龙科技开发有限公司,杭州,330127 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项，国家高技术研究发展计划项目，农业科技成果转化资金项目，浙江省重大科技专项，上海市教育委员会E-研究院建设项目 |
| 摘 要： | 研究了不同保存介质(体腔液CF、Hepes液、RMS液)、温度(4℃、16℃)和保存时间(4 h、8 h、16 h、24 h)对西伯利亚鲟卵子短期保存的影响.结果显示:保存介质、温度和时间对卵子受精率、孵化率和初孵仔鱼畸形率有显著性影响(P<0.05),受精率、孵化率均随保存时间的延长而下降,畸形率上升.16℃条件下保存卵子受精率、孵化率和畸形率均高于4℃,但4℃下卵子的保存时间较16℃下长.研究表明,采用根据西伯利亚鲟体腔液生化成分配制的Hepes液作为保存介质,于16℃下保存4 h为西伯利亚鲟卵子的最佳保存条件,此时受精率、孵化率和畸形率分别为86.36%、94.74%和0. |
| 关 键 词： | 西伯利亚鲟 卵子 保存介质 温度 短期保存 |
Effects of storage medium and temperature on short term storage of |Acipenser baerii eggs. |
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| Affiliation: | Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China|College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, Liaoning, China|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China|Hangzhou Qiandaohu Xunlong Sci Tech Development Co., Ltd, Hangzhou 330127, China |
| Abstract: | This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer’s solution), temperature (4 ℃ and 16℃), and duration (4 h, 8 h, 16 h, and 24 h) on the short term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P<0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 ℃ had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 ℃. The optimal conditions for the short term storage of A. baerii eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 ℃, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained. |
| Keywords: | Acipenser baerii egg storage medium temperature short term storage |
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