Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis |
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| Authors: | R Lazraq S Clavel-Sérès HL David D Roulland-Dussoix |
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| Affiliation: | Department of Pharmaceutical Sciences, University of Bologna, Italy. |
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| Abstract: | Plasmid DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 10(4) transformants/microgram DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformation efficiency. |
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| Keywords: | Bacillus subtilis Transformation Electroporation |
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