Organic solvent tolerance of an alkaline protease from salt-tolerant alkaliphilic <Emphasis Type="Italic">Streptomyces clavuligerus</Emphasis> strain Mit-1 |
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Authors: | Jignasha T Thumar Satya P Singh |
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Affiliation: | (1) Department of Biosciences, Saurashtra University, Rajkot, Gujarat, 360005, India;(2) Department of Microbiology (PG section), M.&N. Virani Science College, Rajkot, Gujarat, India |
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Abstract: | A salt-tolerant alkaliphilic actinomycete, Mit-1 was isolated from Mithapur, coastal region of Gujarat, India. The strain
was identified as Streptomyces clavuligerus and based on 16S rRNA gene sequence (EU146061) homology; it was related to Streptomyces sp. (AY641538.1). The organism could grow with up to 15% salt and pH 11, optimally at 5% and pH 9. It was able to tolerate
and secrete alkaline protease in the presence of a number of organic solvents including xylene, ethanol, acetone, butanol,
benzene and chloroform. Besides, it could also utilize these solvents as the sole source of carbon with significant enzyme
production. However, the organism produced spongy cell mass with all solvents and an orange brown soluble pigment was evident
with benzene and xylene. Further, the enzyme secretion increased by 50-fold in the presence of butanol. With acetone and ethanol;
the enzyme was highly active at 60–80°C and displayed optimum activity at 70°C. The protease was significantly stable and
catalyzed the reaction in the presence of xylene, acetone and butanol. However, ethanol and benzene affected the catalysis
of the enzyme adversely. Crude enzyme preparation was more stable at 37°C in solvents as compared to partially purified and
purified enzymes. The study holds significance as only few salt-tolerant alkaliphilic actinomycetes are explored and information
on their enzymatic potential is still scares. To the best of our knowledge this is the first report on organic solvent tolerant
protease from salt-tolerant alkaliphilic actinomycetes. |
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Keywords: | Alkaline protease Streptomyces sp Streptomyces clavuligerus organic solvent tolerance Non-aqueous catalysis |
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