Effect of anticoagulants in vitro on the viability of lymphocytes and content of free fatty acids in plasma |
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Authors: | Ami Klein Sharon Ramcharitar Nevena Christeff Erik Nisbett-Brown Emmanuel Nunez Aaron Malkin |
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Affiliation: | (1) Dept. of Clinical Biochemistry, Sunnybrook Medical Centre, University of Toronto, 2075 Bayview Avenue, M4N 3M5 Toronto, Ontario, Canada;(2) Department of Biochemistry, Faculte de Medecine Xavier Bichat, Paris, France;(3) Blood Transfusion Services, Canadian Red Cross, Toronto, Canada |
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Abstract: | Summary These authors attempted to test the effect of anticoagulants on lymphocytes viability by reproducing the procedure used for
lymphocyte isolation for various immunologic tests in which blood specimens are allowed to stay at room temperature for 2
h before lymphocytes are isolated. Blood was obtained with three different anticoagulants i.e. heparin, citrate, and CPDA
(citrate, phosphate, dextrose, and adenine). Plasma was lyophilized and extracted with ethanol. Dried ethanol extracts were
suspended in medium (RPMI 1640+10% fetal bovine serum) and incubated with a lymphocyte cell line (MOLT-4). After 24 h of incubation
the viability of cells was examined. The following death rates of the cells were observed: heparin −63±4.6% (mean±SEM), citrate
−27±6.7%, and CPDA 6.2±0.6% (P<0.0005). A significant correlation was found between these results and changes in the concentrations of free fatty acids
in the extracts. These results emphasize the importance of choosing the right anticoagulant when the viability of lymphocytes
is obligatory. |
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Keywords: | fatty acids anticoagulants lymphocyte viability |
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