马拉色菌相关人群及正常人群耳耵聍中马拉色菌带菌情况的调查

目的调查马拉色菌相关人群及正常人群耳耵聍中马拉色菌带菌情况。方法用结晶紫染色法对96例被调查人群耳耵聍进行马拉色菌检测，同时作培养，并以标准株作对照，用生理生化方法将耵聍中分离到的79株马拉色菌进行分类。结果马拉色菌相关人群耳耵聍中马拉色菌的直接检出率为91．84％（45／49），培养阳性率为81．63％（40／49），其中厚皮马拉色菌8株（16．33％），合轴马拉色菌10株（20．41％），糠秕马拉色菌22株（44．90％）。正常人群耳耵聍马拉色菌直接检出率为89．36％（42／47），培养阳性率为78．72％（37／47），其中厚皮马拉色菌5株（10．64％），合轴马拉色菌8株（17．02％），糠秕马拉色菌23株（48．93％），斯洛菲马拉色菌1株（2．13％）。结论马拉色菌为正常人群及马拉色菌相关人群外耳道正常菌群，两组人群中马拉色菌的分离率和菌种分布无显著性差异。

Isolation of Malassezia spp. in cerumen of healthy human and Malassezia associated patients

Objective To investigate the constitution of Malassezia spp.in cerumen of healthy human and Malassezia associated patients.Methods Fourty-seven healthy volunteers and 49 Malassezia associated patients were included.Cerumen was detected by direct microscopic examination using Gram stain,and samples were sent for isolation simultaneously.The standard 7 strains of Malassezia spp.were used as control.The isolates were identified to species by physiological and morphological characeristics.Results In cerumen of Malassezia associated patients,45(91.84%)were positive using direct microscopic examination,40(81.63%)strains were isolated,including 8 strains of Malassezia pachydermatis,10 strains of Malassezia sympodialis,22 strains of Malassezia furfur.In cerumen of healthy volunteers,42(89.36%)were positive using direct microscope examination,37(78.72%)strains were isolated,including 5 strains of Malassezia pachydermatis,8 strains of Malassezia sympodialis,23 strains of Malassezia furfur and 1 strain of Malassezia sloffiae.Conclusions Malassezia spp.were normal commensal in cerumen of human and Malassezia associated patients.No difference of isolation rate and constitution of Malassezia spp.in cerumen were detected in these two groups.