| 响应面法优化红景天总黄酮提取工艺的研究 |
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| 引用本文： | 黄仁福,吴周和,韩春城,朱轶婷.响应面法优化红景天总黄酮提取工艺的研究[J].氨基酸和生物资源,2010,32(1):59-63. |
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| 作者姓名： | 黄仁福 吴周和 韩春城 朱轶婷 |
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| 作者单位： | 湖北工业大学生物工程学院,湖北武汉,430068;湖北工业大学生物工程学院,湖北武汉,430068;湖北工业大学生物工程学院,湖北武汉,430068;湖北工业大学生物工程学院,湖北武汉,430068 |
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| 摘 要： | 研究了红景天中总黄酮提取率的影响因素,先经单因素实验初步确定影响因素,再用Box-Behnken响应面法建立影响因素的二次回归模型,通过对二次回归模型求解编码转换得知,液料比40:1(ml/g),酶添加量1.2%,酶解时间4.5 h,酶解温度45℃,pH5,超声时间为12 min,超声功率为250w中的高档,超声次数为3次,在这最优条件下总黄酮提取率为3.99%。
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| 关 键 词： | 红景天 总黄酮提取率 Box-Behnken 响应面法 |
Optimization of Extraction Technology of Total Flavonoids from Rhodiola sacra by Response Surface Analysis |
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| HUANG Ren-fu,WU Zhou-he,HAN Chun-cheng,ZHU Yi-ting.Optimization of Extraction Technology of Total Flavonoids from Rhodiola sacra by Response Surface Analysis[J].Amino Acids & Biotic Resources,2010,32(1):59-63. |
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| Authors: | HUANG Ren-fu WU Zhou-he HAN Chun-cheng ZHU Yi-ting |
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| Affiliation: | College of Bioengineering;Hubei University of Technology;Wuhan 430068;China |
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| Abstract: | The factors influencing extraction of flavones from Rhodiola sacra were studied by single-factor experiments. Then response surface methodology (RSM)of Box-Behnken was used to optimize the above critical factors. The optimum levels of the factors were calculated through regressive method and the result as follows: samples were hydrolyzed by using the ratio of liquid to material 40:1(ml/g), cellulase of 1.2% , ultrasonic time of 12min for 3 times, ultrasonic powerful strength of 250W,at45℃and pH 5.0 for 4.5hours. Under the optimum levels ,the extraction rate of flavones was up to 3.99%. |
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| Keywords: | Rhodiola sacra extraction rate of flavones Box-Behnken response surface methodology |
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