| 分光光度法测定地骨皮中牛磺酸含量 |
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| 引用本文： | 杨涓,康建宏,魏智清,贠宏伟.分光光度法测定地骨皮中牛磺酸含量[J].氨基酸和生物资源,2006,28(3):26-29. |
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| 作者姓名： | 杨涓 康建宏 魏智清 贠宏伟 |
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| 作者单位： | 宁夏大学,宁夏,银川,750021;宁夏大学,宁夏,银川,750021;宁夏大学,宁夏,银川,750021;宁夏大学,宁夏,银川,750021 |
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| 基金项目： | 宁夏自然科学基金项目(NZ0603)，宁夏大学自然科学基金(NS0506) |
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| 摘 要： | 用分光光度法测定地骨皮中是否含有牛磺酸。在一定条件下,牛磺酸与乙酰丙酮和甲醛反应生成带色的配合物,建立了测定牛磺酸含量的分光光度法。结果表明,地骨皮中含有牛磺酸,已测定样品1中牛磺酸的质量分数为3.124 mg.g-1,样品2中牛磺酸的质量分数为6.203 mg.g-1,且样品2中的牛磺酸质量分数极显著高于样品1(p<0.01)。研究结果表明,地骨皮中含有牛磺酸,而且分光光度法成本低,干扰少,是测定地骨皮中牛磺酸质量分数的较好方法。
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| 关 键 词： | 分光光度法 地骨皮 牛磺酸 |
| 文章编号： | 1006-8376(2006)03-0026-04 |
| 收稿时间： | 05 31 2006 12:00AM |
| 修稿时间： | 2006年5月31日 |
Measurement of Taurine in Lycium Cortex by Ultraviolet Spectrophotometer |
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| YANG Juan,KANG Jian-hong,WEI Zhi-qing,YUN Hong-wei.Measurement of Taurine in Lycium Cortex by Ultraviolet Spectrophotometer[J].Amino Acids & Biotic Resources,2006,28(3):26-29. |
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| Authors: | YANG Juan KANG Jian-hong WEI Zhi-qing YUN Hong-wei |
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| Abstract: | The concentration of taurine in Lycium cortex was measured by ultraviolet spectrophotometer.The method is based on the reaction that taurine reacts with acetyl acetone as well as formaldehyde to produce a stable colored-compound.The results showed that taurine was 3.123mg/g in sample No.1 and 6.196mg/g in sample No.2.If compared with the standard samples, the concentration of taurine in sample No.2 was significantly higher than that of sample No.1(p<0.01).The findings indicated that spectrophotometer was one of the suitable method to detect taurine,which costed less but was effective. |
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| Keywords: | spectrophotometer detection method Lycium cortex taurine |
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