博尔纳病病毒pEGFP-p24基因重组质粒的构建及鉴定

构建博尔纳病病毒pEGFP-p24基因重组表达质粒。通过PCR方法扩增获得博尔纳病痛毒p24基因的完整序列,将此片段定向克隆到pEGFP-N1载体多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用PCR方法和核酸序列测定验证重组质粒构建的正确性。PCR及核酸序列测定证明博尔纳病病毒pEGFP-p24基因重组表达质粒构建成功。构建的重组质粒将为研究博尔纳病病毒p24基因在真核细胞中的功能和作用提供实验依据。

Construction and Identification of Recombinant Plasmid pEGEFP-BDV p24

Whole sequence of p24 gene of Borna disease virus(BDV)was obtained through PCR amplification and inserted this fragment cloned into pulyclonal site domain of vector pEGFP-N1 to construct a plasmid expressing pEG- FP-BDV p24 gene by screening the positive recombinant strain,then extract the recombinant plasmid and proved the validity of the successfully constructed recombinant plasmid with PCR and DNA sequencing.The constructed recombi- nant plasmid will provide experiment foundation to study the function and action of BDV p24 gene in eukaryotic cells.