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低温纤维素降解菌的筛选及其酶学性质初步研究
引用本文:张淑红,刘秀花,梁峰,王莉,刘源慧,翟兴礼,赵龙飞.低温纤维素降解菌的筛选及其酶学性质初步研究[J].微生物学杂志,2009,29(3):97-100.
作者姓名:张淑红  刘秀花  梁峰  王莉  刘源慧  翟兴礼  赵龙飞
作者单位:商丘师范学院,生命科学系,河南,商丘,476000
基金项目:河南省科技攻关项目,河南省重点科技攻关项目 
摘    要:从青藏高原冰川雪样恢复出的4株细菌中筛选出1株降解纤维素能力比较高的菌株LHG-C-9。经16SrDNA序列分析,初步鉴定为假单胞菌属。对该菌所产纤维素酶的性质进行了初步研究,其最适作用温度为30℃;对热敏感;最适pH8.0;属碱性温酶。该低温纤维素酶在纺织、造纸、环保、医药和饲料等行业可望有广泛的应用前景。

关 键 词:低温纤维素酶  细菌  筛选  酶学性质

Screening of Cryophilic Cellulose-Degradable Bacterium and Its Zymological Properties
ZHANG Shu-hong,LIU Xiu-hu,LIANG Feng,WANG Li,LIU Yuan-hui,ZHAI Xing-li,ZHAO Long-fei.Screening of Cryophilic Cellulose-Degradable Bacterium and Its Zymological Properties[J].Journal of Microbiology,2009,29(3):97-100.
Authors:ZHANG Shu-hong  LIU Xiu-hu  LIANG Feng  WANG Li  LIU Yuan-hui  ZHAI Xing-li  ZHAO Long-fei
Affiliation:(Dept. of Life Sci. , Shangqiu Normal Univ., Shangqiu, Henan 476000)
Abstract:A strain LHG-C-9 with high cellulose degradation ability was screened from four bacterial strains recovered from snow samples collected in Qinghai-Tibet plateau glacier. Initial identification indicated that it belonged to Pseudomonas sp. through its 16S rDNA sequence analysis. The initial study on its properties showed that the most suitable temperature of the cellulase was 30 ℃; Sensitive to heat, the most suitable pH value was 8.0, and belonged to alkaline cryo-enzyme. It is hopeful and has wide application vista in textile, paper making, environment protection, medicine, forage, and other industries.
Keywords:cryo-cellulase  bacteria  screening  zymological properties
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