| 接合转移诱动系统在遗传分析和体内基因操作中的应用 |
| |
| 引用本文： | 赵巍,张成刚.接合转移诱动系统在遗传分析和体内基因操作中的应用[J].微生物学杂志,1994,14(2):1-11. |
| |
| 作者姓名： | 赵巍 张成刚 |
| |
| 作者单位： | 中国科学院沈阳应用生态研究所 |
| |
| 摘 要： | 接合转移诱动系统在遗传分析和体内基因操作中的应用赵巍，张成刚，蔺继尚（中国科学院沈阳应用生态研究所，１１００１５）细菌间ＤＮＡ的转移主要有转化、转导、接合和原生质体融合等几种形式。接合是通过供体菌和受体菌完整细胞间的直接接触，而传递大段ＤＮＡ的方法，...
|
| 关 键 词： | 接合 转移 诱动系统 遗传分析 基因操作 细菌 |
IN-VIVO MANIPULATIONS AND GENETIC ANALYSES BY CONJUGAL TRANSFER MOBILIZING SYSTEM |
| |
| zhao wei et al..IN-VIVO MANIPULATIONS AND GENETIC ANALYSES BY CONJUGAL TRANSFER MOBILIZING SYSTEM[J].Journal of Microbiology,1994,14(2):1-11. |
| |
| Authors: | zhao wei |
| |
| Abstract: | Conjugal transfer mobilizing system is an effective tool which can facilitate the transfer of DNA between cells. The vector plasmids and mobilizing strains which compose the conjugal transfer mobilizing system were constructed from oriT and Tra by means of broad host range mibilizable plasmid This system can be widely used for the genetic analyses and manipulationso of bacteria, yeast and plants. This paper summarizes the principles and procedures of the construction of this system, and covers the developments and applications of the system. The range in which the system can be used is also discussed. |
| |
| Keywords: | conjugal transfer mobilizing system broad host range mobilizableplasmid Mob-site in-vivo manipulation genetic analysis |
| 本文献已被 CNKI 维普 等数据库收录！ |
