| 果胶酶高产菌株的紫外线及硫酸二乙酯的诱变育种 |
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| 引用本文： | 由田,宋刚,凌红志,葛菁萍.果胶酶高产菌株的紫外线及硫酸二乙酯的诱变育种[J].微生物学杂志,2011,31(5):69-72. |
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| 作者姓名： | 由田 宋刚 凌红志 葛菁萍 |
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| 作者单位： | 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,黑龙江哈尔滨,150080 |
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| 基金项目： | 黑龙江省博士后启动基金，黑龙江省人事厅留学回国择优资助项目，黑龙江省教育厅振兴老工业基地成果转化项目，黑龙江大学高层次人才(创新团队)支持计划 |
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| 摘 要： | 以HDYM-02为出发菌株，用紫外线及硫酸二乙酯进行诱变，从大量突变株中进行筛选，选育出2株产果胶酶性质稳定且酶活明显提高的突变株UV-21和DES-1，株相比其产酶期提前，前者在24h时的酶活力为出发菌株的1．6倍，后者在12h的酶活力为出发菌株的1．44倍。
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| 关 键 词： | 果胶酶 紫外线 硫酸二乙酯 诱变 |
Mutagenesis Breeding of Pectinase High Producing Strains by UV and DES |
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| YOU Tian,SONG Gang,LING Hong-zhi,GE Jing-ping.Mutagenesis Breeding of Pectinase High Producing Strains by UV and DES[J].Journal of Microbiology,2011,31(5):69-72. |
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| Authors: | YOU Tian SONG Gang LING Hong-zhi GE Jing-ping |
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| Affiliation: | YOU Tian,SONG Gang,LING Hong-zhi,GE Jing-ping(Key Lab.of Microbiol.,Coll.of Life Sci.,Heilongjiang Uni.,Harbin 150080) |
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| Abstract: | Two mutant strains UV-21 and DES-1 were screened from a large number of mutant strains by mutating the original strain HDYM-02 with UV and DES(diethyl sulfate) respectively.Both mutant strains stably yielded pectinase with obvious enhanced pectinase activities.Comparison of the pectinase production time revealed that both mutant strains were earlier than the original one,the former was 1.6 times as high as that of the original strain at 24 h and the latter was 1.44 times as high as that of the original stra... |
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| Keywords: | pectinase UV diethyl sulfate mutagenesis |
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