Biodegradation of 4-aminobenzenesulphonate by a newly isolated bacterial strain PNS-1 |
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Authors: | Poonam?Singh LC?Mishra Email author" target="_blank">Leela?IyengarEmail author |
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Affiliation: | (1) Department of Chemistry, Biotechnology Laboratory, Indian Institute of Technology, Kanpur, India, 208016;(2) Department of Life Sciences, C.S.J.M. University, Kanpur, India, 208016 |
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Abstract: | A bacterial strain PNS-1, isolated from activated sludge derived from a domestic wastewater treatment unit, could utilize 4-aminobenzenesulphonate (4-ABS) as a sole organic carbon and energy source under aerobic conditions. Degradation rate varied with the initial concentration of 4-ABS and maximum specific substrate removal rate was observed at 400mg 4-ABS l–1 (2.3mM). Average biomass yield was 0.31mg/mg 4-ABS degraded. Biokinetic parameters for the degradation, determined using the Haldane relationship, were 0.26h–1 (max), 6mg\,l–1 (KS) and 4020mg\,l–1 (Ki). Strain PNS-1 could not utilize other isomers of benzenesulphonate and 5-sulphosalicylate as growth substrates whereas protocatechuate, pyrocatechuate and p-hydroxybenzoate could be degraded. In mixed substrate batch cultivations, where 4-ABS was one of the component, protocatechuate and 4-ABS were simultaneously utilized. Presence of 2- or 3-ABS decreased the growth and substrate degradation rates of 4-ABS. With 4-ABS and pyrocatechuate, although a lag phase was observed prior to pyrocatechuate degradation, a diauxic growth pattern was not seen. |
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Keywords: | Aminobenzenesulphonate biodegradation xenobiotics kinetic parameters sulphanilic acid |
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