改良颈静脉置管术在自身给药成瘾模型优化中的作用研究

目的:探讨技术和方法的改进,提高动物颈静脉置管以及药物成瘾自身给药模型构建的成功率,为成瘾研究提供更稳定和高效的建模方法。方法:对建立自身给药模型的传统颈静脉置管术进行改良,选取成年雄性SD大鼠60只,按照随机数字表法分为传统手术组(n=30)和改良手术组(n=30),分别完成颈静脉置管术后,按照随机数字表法,再将每组分为对照训练组(n=15)和成瘾训练组(n=15),构建大鼠自身给药模型,观察两组大鼠自身给药模型成功率。结果:大鼠的颈静脉置管手术可能出现的手术并发症主要包括堵管、漏管、感染甚至死亡等,最主要的并发症是漏管,占比最大。颈静脉置管传统手术组手术成功率为43.33%±3.333,颈静脉置管改良手术组手术成功率为90.00%±3.333,显著高于颈静脉置管传统手术组(P0.05)。两组成瘾训练组有效鼻触次数均分别明显高于其对照训练组(P0.05)。结论:大鼠改良后的颈静脉置管手术效果明显优于传统手术,颈静脉置管手术成功率明显提高。

The Role of Modified Jugular Vein Catheterization in the Optimization of Self-administration Model for Drug Addiction

ABSTRACT Objective: To improve the success rate of animal jugular vein catheterization and self-administration model of drug addiction via probing the optimization of technique and method, and provide more stable and efficient modeling solutions for drug addiction research. Methods: The traditional jugular vein catheterization for constructing self-administration model was optimized, 60 adult male Sprague-Dawley rats were selected and divided into two groups by random number table method, including the traditional operation group (n=30) and the modified operation group (n=30). After completion of jugular vein catheterization, both groups of rats were individually divided into the control training group (n=15) and the addictive training group (n=15) in the same method. The success rate of jugular vein catheterization in self-administration model of rats were observed and analyzed. Results: The possible surgical complications of jugular vein catheterization for rats included catheter blocking, catheter leakage, infection and even death, and the catheter leakage acted as the most frequent type among all kinds of complications. The success rate of operation in traditional operation group was 43.33%±3.333, which was significantly higher than that in traditional operation group (90.00%±3.333, P<0.05). The effective nose poking times for both addiction training groups were significantly higher than those of the two control training groups respectively (P<0.05). Conclusion: The surgical efficacy of modified jugular vein catheterization for rats was significantly better than the traditional operation, the success rate of jugular vein catheterization operation was significantly enhanced.