皮下种植黑色素瘤对小鼠血液中脂肪和氨基酸代谢的影响*

目的:探讨皮下种植黑色素瘤对小鼠血液中相关脂肪和氨基酸代谢的影响。方法:野生型小鼠皮下种植B16黑色素瘤细胞,两周后观察小鼠并取不同的组织称重,检测血液中甘油三酯、葡萄糖、脂肪酸、炎症因子的水平。利用质谱方法检测血液中氨基酸的水平。结果:野生型小鼠皮下肿瘤种植2周后体重没有明显变化,脾脏和肝脏的重量明显增加,皮下脂肪组织和腹腔脂肪组织的重量没有明显变化。血液中甘油三酯的水平明显增高,葡萄糖和甘油的水平明显下降。血液中炎症因子Interleukin 6(IL6)和Interferon gamma(IFNγ)的水平明显增加。血液的质谱结果显示有四种氨基酸的水平有明显变化,其中胱氨酸(Cystine)增加了约30倍,丝氨酸(Serine)、谷氨酰胺(Glutamine)和色氨酸(Tryptophan)分别有不同程度的降低。结论:皮下黑色素瘤显著影响小鼠机体营养状态和炎症,并特异性影响血液中氨基酸的代谢。

The Effect of Subcutaneous B16 Melanoma on the Blood Lipid and Amino Acid Metabolism in Mice*

ABSTRACT Objective: Here, we want to investigate the role of subcutaneous B16 melanoma in regulating blood lipid and amino acid metabolism in mice. Methods: The mice were inoculated subcutaneously with B16 melanoma. Two weeks later, mice were sacrificed and tissues were collected. The weight of adipose tissue, liver and spleen were measured. Serum level of triglyceride, glucose glycerol and free fatty acids were measured. Serum metabolite changes of amino acids were detected by targeted metabonomic analysis. Results: The body weight of 2 week-B16-inoculated mice was similar to control mice. The livers and spleens of B16-inoculated mice were larger than that of control mice. Regardless of no changes in the weight of subcutaneous and gonadal fat tissues. The serum level of triglyceride was increased in B16-inoculated mice, while the level of glucose and glycerol was decreased. Serum level of Cystine was increased 30 folds in B16-inoculated mice compared with control mice. Level of Serine, Glutamine and Tryptophan was decreased in B16-inoculated mice. Conclusions: Subcutaneous B16 inoculation in mice dramatically affected the nutrition and inflammation status of mice. B16-inoculation in mice altered specific amino acids level in serum.