A conserved sequence in caveolin-1 is both necessary and sufficient for caveolin polarity and cell directional migration |
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Authors: | Xing-Hui Sun Hong Chen Qi Qi |
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Affiliation: | a Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506, USA b Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV 26506, USA c Department of Radiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA |
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Abstract: | Caveolin-1 (Cav-1) plays an important role in the organization of signaling molecules involved in a variety of signaling pathways, including those mediating cell motility. Here we show that amino acids K47-K57 of Cav-1 are a highly conserved sequence in Cav-1 and Cav-3 proteins, and that expression of either K47-K57 deletion Cav-1 mutant or wild-type Cav-2 that lacks this sequence exhibits a non-polarized distribution pattern. Expression of K47-K57 in Cav-2 leads to Cav-2 polarity, suggesting that expression of K47-K57 is sufficient to direct caveolin polarity. Importantly, we show that expression of this sequence is both necessary and sufficient to promote cell directional migration. Thus, our results support the conclusion that Cav-1 polarity is critical for cell directional migration. |
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Keywords: | Cav-1 caveolin-1 CPD caveolin polarization domain Cav-2 caveolin-2 aa amino acids MEFs mouse embryonic fibroblasts WT wild-type KO knockout GAPDH glyceraldehyse-3-phosphate dehydrogenase |
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