广东石豆兰多糖的提取工艺及其抗氧化活性

为优化广东石豆兰多糖的提取工艺并评价其抗氧化活性。在单因素试验基础上,采用正交设计和方差分析,研究液料比、提取温度、时间及沉淀多糖的乙醇浓度对石豆兰多糖浸提量的影响并优化了工艺条件,确定最优条件为液料比50∶1 (mL/g),提取温度90℃,时间6 h,醇沉浓度为95%,此条件下多糖提取量为79.060 mg/g,RSD为0.132%。抗氧化结果表明广东石豆兰多糖的总还原力为L-抗坏血酸的3.46%;对DPPH、·OH、·O■自由基半清除浓度(EC₅₀)分别为2769.58、594.60、586.94μg/mL,其清除能力分别是L-抗坏血酸的0.68%、47.17%和29.00%;对Fe²⁺的半螯合浓度(EC₅₀)为160.83μg/mL,螯合能力是EDTA的2.12%。本研究结果为石豆兰多糖的提取及进一步开发利用提供参考依据。

Optimization of the extraction process of polysaccharide from Bublophyllum kwangtungense and its antioxidant activity

In this experiment,optimization of extraction process of polysaccharide from Bublophyllum kwangtungense and its antioxidant activity were studied. On the basis of single factors experiments,orthogonal design and variance analysis were ul- tilized to investigate the effects of liquid-to-solid,extraction temperature and time,ethanol concentration for precipitation on the content of polysaccharide,and optimize the process. The optimal extraction conditions were determined as follows∶ liquid- to-solid,50∶ 1( mL/g);extraction temperature,90 ℃,and extraction time,6 h;ethanol heavy concentration,95%. Under the optimal conditions,the yield of polysaccharide of 79. 060 mg /g was gained,and the RSD value was 0. 132%. Polysaccharide displays good antioxidant activity with that total reducing power is 0. 035 times of L-ascorbic acid,and the EC50 values for DP- PH,hydroxyl radicals and superoxide anions are 2769. 58,594. 60,586. 94 μg /mL,and the cleanrance ability are 0. 68%, 47. 17% and 29. 00% of L-ascorbic acid respectively. The chelating rate to Fe2 + with EC50 values of 160. 83 μg /mL,and the chelating ability is 2. 12% of EDTA. These results provided a reference for the extraction,further development and ultilization of the polysaccharide in B. kwangtungense.