均匀设计法优化高丽参多糖的提取

利用均匀设计法优化了高丽参多糖的提取过程.主要考察料液质量比、浸泡时间、提取温度和提取时间四个因素对提取率的影响.通过数学模型得到最优提取状况和3D曲面图.结果表明,提取温度对高丽参多糖得率影响最大,在料液质量比1:35、浸泡时间135 min、水提取温度81℃条件下提取156 min,高丽参多糖最大理论提取率为49.51%.最优化模型下实际多糖得率49.29%与理论得率相符.

Optimization on the Extraction of Polysaccharides from Ginseng Using Uniform Design

Uniform Design was used to optimize the effects of processing parameters for extraction of polysaccharides from ginseng. Four independent variables including extraction time (min), solid:liquid ratio(g/mL), immersing time (min), and extraction temperature ( ℃ ) were study. The optimal conditions were determined and tri-dimensional response sur- faces were plotted according to the mathematical models. The results indicated that extraction temperature variables markedly affect the polysaccharides yield. The predicted optimal polysaccharides yield was 49.51% under the optimum conditions:liquid:solid ratio of 1:35 ,immersing time of 135 min, water extraction temperature of 81 ℃ ,and water ex- traction time of 156 min. Under optimized conditions the experimental yield (49.29%) in agreement with the predicted yield.