微水相超声波协同固定化脂肪酶催化酯交换过程优化

超声波协同固定化脂肪酶催化制备生物柴油的最佳工艺条件为：超声波功率70W、叔丁醇为反应介质、叔丁醇用量3％（v／v）、醇油比3：1且甲醇分三批加入、反应温度40℃、水含量为2％（v／v）。副产物甘油对固定化脂肪酶使用寿命影响最大，使用后的固定化脂肪酶用丙酮洗去表面的甘油，进行酯交换反应，酶的稳定性大为提高，可连续使用16批次。

Optimized Process of Biodiesel on Immobilized Lipase Catalyst Assisted with Ultrasonic Radialization in Microaqueous Media

Assisted with ultrasonic radialization in raicroaqueous media,the optimized conditions of preparation biodiesel catalyzed by the immobilized lipase were confirmed. The optimized conditions,such as ultrasonic power 70 W,n-butyl alcohol amount 3% (v/v of oil),molar ratio of the methanol to oil 3:1,40℃ , water 2% (v/v of oil) when adding metha-nol according to the same proportions three times,obtained a maximum ester yield 98.6%. By-product glycerol was the key point to influence the catalyst service life. If removing the glycerol on the surface of the immobilized lipase,it could be used for 16 times.