取食转Bt基因水稻对稻纵卷叶螟幼虫中肠的组织病理学效应

利用透射电镜观察了稻纵卷叶螟 Cnaphalocrocis medinalis（Guenée）幼虫取食转Bt基因水稻后中肠的组织病理变化。结果表明：稻纵卷叶螟幼虫取食转cry1Ab基因水稻后,中肠上皮细胞的线粒体先发生形态变化,随连续取食时间的延长线粒体出现凝聚、内嵴稀疏、空泡化等,在后期还呈凝聚态随突起脱落或沿杯腔边沿单一排列。内质网的变化也很明显,病变过程中伴随着粗糙内质网的肿胀、核糖体脱落,粗糙内质网增多等现象。细胞核的变化较小,在处理后期出现细胞核拉长、核仁聚集等变化。组织病变程度不一,有的细胞在病变早期就出现了空泡化。

Histopathological changes in the midgut of the rice leaf folder,Cnaphalocrocis medinalis (Lepidoptera: Pyralidae ) larvae feeding on transgenic Bt rice

The histopathological changes of the midgut of rice leaf folder, Cnaphalocrocis medinalis (Guenée) larvae feeding on Bt rice line“KMD1" and“KMD2" were observed using transmission electron microscopy. The results showed that cellular alternations in the midgut tissues, such as swelling of mitochondria, endoplasmic reticulum, and dilatation of intercellular spaces, fenestration or disorganization of the Golgi complex, and concentric arrangement of rough endoplasmic reticulum. The initial infected organelles were mitochondria. After longer feeding on Bt rice by the larvae, the upper portions of the cells swelled, and cytoplasm in these regions decreased in electronic density and other organelles were damaged to a certain degree, such as wiredraw of nucleus, condensation of nuclear, and the increase of number of rough endoplasmic reticulum. As the infection proceeded further, pathological changes in the mitochondrial abundant areas of basal membrane enfoldings of columnar cells and cytoplasmic projections of goblet cells became more obvious. Finally, the midgut epithelium cells disrupted and sloughed off from the basal membrane.