| 柘叶饲养对家蚕消化液中抗核多角体病毒（BmNPV）相关蛋白活性的影响 |
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| 引用本文： | 王晓强,冯伟,谢洪霞,周围,张冉,高红,万永继.柘叶饲养对家蚕消化液中抗核多角体病毒（BmNPV）相关蛋白活性的影响[J].昆虫学报,2011,54(4):404-408. |
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| 作者姓名： | 王晓强 冯伟 谢洪霞 周围 张冉 高红 万永继 |
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| 作者单位： | 西南大学生物技术学院, 重庆北碚 400716 |
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| 基金项目： | 公益性行业(农业)科研专项，国家现代农业产业技术体系建设专项 |
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| 摘 要： | 为探讨柘叶Cudrania tricuspidata饲养家蚕Bombyx mori易感染核多角体病毒（BmNPV）的机制, 本实验比较研究了分别以柘叶和桑叶Morus alba饲养家蚕后其消化液中抗病毒蛋白活性的差异。结果表明: 家蚕经柘叶饲养后消化液中红色荧光蛋白（red fluorescent protein, RFP）的强度无明显变化, 但柘叶饲养蚕消化液中脂肪酶、 胰蛋白酶的活性显著低于桑叶饲养蚕, 柘叶饲养蚕消化液中脂肪酶和胰蛋白酶的活性分别为1 421.71±202.60 U/L和19.67±8.17 U/mL, 桑叶饲养蚕脂肪酶和胰蛋白酶的活性分别为1 976.03±139.92 U/L和199.18±181.71 U/mL。这些结果说明, 消化液中脂肪酶和胰蛋白酶的活性水平低与柘叶饲养蚕易感染核型多角体病毒（BmNPV）可能相关。
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| 关 键 词： | 家蚕 柘树 家蚕核多角体病毒 红色荧光蛋白 脂肪酶 胰蛋白酶 |
| 收稿时间： | 2010-08-29; |
Activities of anti-BmNPV proteins in digestive juice of the silkworm (Bombyx mori)reared on cudrang(Cudrania tricuspidata)leaves |
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| WANG Xiao-Qiang,FENG Wei,XIE Hong-Xia,ZHOU Wei,ZHANG Ran,GAO Hong,WAN Yong-Ji.Activities of anti-BmNPV proteins in digestive juice of the silkworm (Bombyx mori)reared on cudrang(Cudrania tricuspidata)leaves[J].Acta Entomologica Sinica,2011,54(4):404-408. |
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| Authors: | WANG Xiao-Qiang FENG Wei XIE Hong-Xia ZHOU Wei ZHANG Ran GAO Hong WAN Yong-Ji |
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| Affiliation: | College of Biotechnology, Southwest University, Beibei, Chongqing 400716, China |
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| Abstract: | It had been reported that silkworms Bombyx mori reared on cudrang (Cudrania tricuspidata) leaves are sensitive to B. mori nucleopolyhedrovirus (BmNPV). In order to explore the mechanisms of this phenomenon,we investigated the activities of different anti-BmNPV proteins in digestive juice of the silkworm reared on cudrang leaves and mulberry (Morus alba) leaves,respectively. The results showed that there was no significant change in intensity of red fluorescent protein (RFP) in the digestive juice of the sil... |
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| Keywords: | Bombyx mori Cudrania tricuspidata BmNPV RFP lipase trypsin |
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