Adsorption/Desorption Behavior of Bovine Serum Albumin and Porcine Insulin on Chemically Patterned Porous Gel Networks |
| |
Authors: | Herman S Mansur Rodrigo L Oréfice Zélia P Lobato Wander L Vasconcelos Eliane S Mansur Lucas J C Machado |
| |
Affiliation: | (1) Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Rua Espírito Santo, 35/2 andar, 30160.030 Centro, Belo Horizonte, MG, Brazil |
| |
Abstract: | Adsorption/desorption of proteins onto a biomaterial surface plays a major role on the biocompatibility of the implanted material. By modifying the biomaterial surface with specially designed functional groups one may achieve the most specific behavior of the developed material used in a biological system. Based on that, porous gel matrixes with functionalized surfaces offer unlimited possibilities to control the protein-substrate interaction behavior. In the present work, we have functionalized the surface of porous glass with several chemical groups during the synthesis of the silica matrix. The porous glass matrixes were obtained using tetraethoxysilane (TEOS)/ethanol and functionalized with 3-mercaptopropyltrimethoxysilane (MPTMS) and 3-aminopropyltriethoxysilane (APTES). In vitro tests of the kinetics of protein adsorption and desorption from the gel matrix were monitored by UV-visible spectroscopy. The bioactivity of the incorporated protein was verified by in vivo experiments with adult male rats, where they presented an acute hypoglycemic peak. |
| |
Keywords: | protein adsorption adsorption kinetics sol-gel process porous glass surface engineering |
本文献已被 SpringerLink 等数据库收录! |