高效抗菌Cu~(2+)-壳聚糖季铵盐/丙烯酸复合物的制备

以过硫酸铵为引发剂,在壳聚糖季铵盐(HACC)上接枝丙烯酸(AAc)并络合Cu~(2+),制备了具有高效抗菌性能的HACC-g-PAAc-Cu~(2+)复合物.采用红外光谱(FTIR)和核磁共振波谱(~1H NMR)表征了壳聚糖季铵盐接枝改性前后的化学结构变化;利用紫外-可见吸收光谱(UV-Vis)、Cu~(2+)选择电极和热失重分析(TGA)表征了壳聚糖季铵盐接枝前后负载Cu~(2+)的能力;测定了HACC-Cu~(2+)和HACC-g-PAAc-Cu~(2+)对金黄色葡萄球菌及大肠杆菌的最小抑菌浓度,并对小鼠经口摄入毒性和兔皮肤敷涂刺激性进行了考察.研究结果表明,在壳聚糖季铵盐上负载Cu~(2+)能够有效提高其抗菌性;接枝丙烯酸能提高HACC负载Cu~(2+)的能力和抗菌性,Cu/HACC结构单元的摩尔比由接枝前的3∶7提高到接枝后的1∶1;对金黄色葡萄球菌的最小抑菌浓度由60 mg/L下降到9.2 mg/L,对大肠杆菌的最小抑菌浓度由37 mg/L下降到6.3 mg/L,无摄入毒性和皮肤刺激性.

Preparation of Cu2+-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity

Acrylic acid(AAc) was grafted to chitosan quaternary ammonium salt(HACC) by ammonium persulfate as an initiator.HACC-g-PAAc-Cu2+ with efficient antibacterial activity was further prepared through complexation of Cu2+ with HACC-g-PAAc.The chemical structure of the grafted HACC was characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance(1H NMR).The capacity of grafted HACC loading Cu2+ was determined by ultraviolet-visible spectroscopy(UV-Vis) and thermagravimetric analysis(TGA).Antimicrobial property of HACC-Cu2+ and HACC-g-PAAc-Cu2+ against %staphylococcus aurous(S.Aurous%) and %E.coli% bacillus were tested.Toxicity for oral intake of mice and coating irritation on rabbit skin of HACC-Cu2+ and HACC-g-PAAc-Cu2+ were also evaluated.The property of antibacterial and capacity of complexing Cu2+ can be improved by grafting of AAc.The molar ratio of Cu2+/HACC increased from 3∶7 before grafting to 1∶1 after grafting.The minimum inhibitory concentration(MIC) of %S.Aurous% and %E.coli% bacillus decreased from 60 mg/L to 9.2 mg/L and 37 mg/L to 6.3 mg/L.Moreover, HACC-Cu2+ and HACC-g-PAAc-Cu2+ have no toxic ingestion and skin irritation.