HPLC determination of colistin and aminoglycoside antibiotics in feeds by post-column derivatization and fluorescence detection |
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Authors: | Gy Morovján P P Csokán L Németh-Konda |
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Affiliation: | (1) State Institute for the Control of Veterinary Biologicals, Drugs and Feeds, 8 Szállás str., 1107 Budapest, Hungary |
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Abstract: | Summary Reversed-phase, HPLC methods employing post-column derivatization and fluorescence detection were developed for the determination
of the peptide colistin and four aminoglycoside antibiotics in feeds. Extraction of the analytes was by sonication and shaking
with dilute hydrochloric acid. Post-column derivatization was performed using orthophtaldialdehyde-2-mercaptoethanol chemistry.
Assay of colistin was by using an acetonitrile-aqueous sodium sulphate-triethylammonium phosphate (pH 2.8) eluent. Aminoglycoside
antibiotics:amikacin, kanamycin, gentamycin and neomycin were analyzed using a tetrahydrofuran-aqueous sodium sulphate-sodium
pentanesulphonate-acetic acid mobile phase. The method was also applied to some pharmaceutical preparations. Preliminary results
showed that the method can be adapted for the assay of the above antibiotics in meat and animal serum for residue and pharmacokinetic
studies.
Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997. |
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Keywords: | Column liquid chromatography Post-column derivatization Feeds Aminoglycoside antibiotics Colistin |
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