Bienzyme sensors based on novel polymethylferrocenyl dendrimers |
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| Authors: | J Losada M Zamora P García Armada I Cuadrado B Alonso C M Casado |
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| Affiliation: | (1) Dpto. de Ingeniería Química Industrial, E. T. S. I. I., Universidad Politecnica Madrid, José Gutiérrez Abascal n° 2, 28006 Madrid, Spain;(2) Dpto. de Química Inorgánica, Facultad de Ciencias, U. A. M., Cantoblanco, 28049 Madrid, Spain |
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| Abstract: | Amperometric bienzyme electrodes with horseradish peroxidase (HRP) and glucose oxidase (GOx) co-immobilized on polymethylferrocenyl
dendrimers deposited onto platinum electrodes have been used for determination of the hydrogen peroxide produced by the oxidase
during the enzymatic reaction. The redox dendrimers consist of flexible poly(propylenimine) dendrimer cores functionalised
with octamethylferrocenyl units. The effects of dendrimer generation, the thickness of the dendrimer layer, substrate concentration,
interferences, and reproducibility on the response of the sensors were investigated. The new bienzyme biosensors respond to
substrate at work potential values between 200 and 50 mV (vs. SCE), have good sensitivity, and are resistant to interferences.
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| Keywords: | Polymethylferrocene Dendrimers Bienzyme electrodes Horseradish peroxidase (HRP) Glucose oxidase Amperometric biosensor |
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