| 可控组装方法制备脂质体 |
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| 引用本文： | 张林刚,胡继存,陆祖宏.可控组装方法制备脂质体[J].化学学报,1998,56(4):335-339. |
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| 作者姓名： | 张林刚 胡继存 陆祖宏 |
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| 作者单位： | 东南大学分子与生物分子电子学实验室,南京,210096 |
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| 摘 要： | 利用可控组装方法制备了单仓脂质体。这种制备方法分为两个步骤: (a)利用超声振荡形成油包水(W/O)的微乳液, (b)微乳中小水滴在离心作用下通过油水界面的单分子膜并被其包裹, 在水相中形成脂质体。脂质体直径在50-200nm。脂质体的双分子膜间基本没有残留有机溶剂存在, 并且在制备过程中包封的试剂没有泄漏。可控组装方法制备脂质体具有操作简单、生成脂质体一般不需进一步分离、包封效率较高(57%)等特点。
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| 关 键 词： | 脂质体 微乳 囊泡 |
| 修稿时间： | 1996年9月17日 |
Preparation of liposomes by a controlled assembling method |
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| ZHANG Lin-gang,HU Ji-chun,LU Zu-hong.Preparation of liposomes by a controlled assembling method[J].Acta Chimica Sinica,1998,56(4):335-339. |
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| Authors: | ZHANG Lin-gang HU Ji-chun LU Zu-hong |
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| Abstract: | A method of liposome preparation is demonstrated, which includes two experimental steps: (a) water in oil (W/O) microemulsion are prepared by sonication; (b) water droplets in the W/O microemulsion pass through the monolayer at the oil - water interface by centrif ugation and transform into liposomes. Sizes of liposomes are 50 ~ 200nm in diameter. The percentage capture reached 57% and no leakage of encapsulated content was observed during the preparation. |
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| Keywords: | liposome vesicle W/O emulsion |
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