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Dynamic variations of bioactive compounds driven by enzymes in Psoralea corylifolia L. from growth to storage and processing |
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| Affiliation: | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
| Abstract: | Fructus Psoraleae (FP), the dried ripe fruit of Psoralea corylifolia L., is a popular herbal medicine commonly applied for alleviating osteoporosis and vitiligo. But, until now, the dynamic variations of compounds in P. corylifolia have been less investigated during its growth, storage, and treatment by different temperatures, which is meaningful for guaranteeing the quality of FP. In this study, focused on these questions, with emphasis on the enzyme-driven dynamic transformation of coumarins, ultra-high performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA) method was successfully established for the simultaneous determination of nine compounds. The distribution and accumulation of compounds were discussed and illuminated in different parts of P. corylifolia and samples harvested at different times. The characteristics of compounds' variation in flowers and fruits of P. corylifolia were identified. Through the market survey and quantitative study on FP, positive correlation was speculated between transformation from (iso)psoralenoside to (iso)psoralen via β-glucosidase and storage time, which was further confirmed by accelerated stability test. The effect of treated temperatures (40–210 °C) was unveiled on the enzyme activity and transformation from (iso)psoralenoside to (iso)psoralen in FP. And the focused compounds' transformation was mainly driven by β-glucosidase when the temperature was below 120 °C. Above 120 °C, β-glucosidase was completely inactivated, and the focused compounds' transformation was mediated by high-temperature, also the obvious degradation was found. Our results demonstrated that compounds' transformation characteristics arising from the growth, processing and storage of P. corylifolia are critical factors to ensure the quality of FP. |
| Keywords: | Fructus Psoraleae Enzyme Growth Storage Processing FP"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"Fructus Psoraleae PO"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"psoralenoside IPO"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"isopsoralenoside (I)PO"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"(iso)psoralenoside P"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"psoralen IP"} {"#name":"keyword" "$":{"id":"k0090"} "$$":[{"#name":"text" "_":"isopsoralen (I)P"} {"#name":"keyword" "$":{"id":"k0100"} "$$":[{"#name":"text" "_":"(iso)psoralen Neo"} {"#name":"keyword" "$":{"id":"k0110"} "$$":[{"#name":"text" "_":"neobavaisoflavone Iso"} {"#name":"keyword" "$":{"id":"k0120"} "$$":[{"#name":"text" "_":"isobavachalcone Pso"} {"#name":"keyword" "$":{"id":"k0130"} "$$":[{"#name":"text" "_":"psoralidin Bav"} {"#name":"keyword" "$":{"id":"k0140"} "$$":[{"#name":"text" "_":"bavachinin Bak"} {"#name":"keyword" "$":{"id":"k0150"} "$$":[{"#name":"text" "_":"bakuchiol LC-MS/MS"} {"#name":"keyword" "$":{"id":"k0160"} "$$":[{"#name":"text" "_":"liquid chromatography-tandem mass spectrometry UHPLC-PDA"} {"#name":"keyword" "$":{"id":"k0170"} "$$":[{"#name":"text" "_":"ultra-high performance liquid chromatography with photo-diode array detector LOD"} {"#name":"keyword" "$":{"id":"k0180"} "$$":[{"#name":"text" "_":"limit of detection LOQ"} {"#name":"keyword" "$":{"id":"k0190"} "$$":[{"#name":"text" "_":"limit of quantification S/N"} {"#name":"keyword" "$":{"id":"k0200"} "$$":[{"#name":"text" "_":"signal to noise ratio ChP"} {"#name":"keyword" "$":{"id":"k0210"} "$$":[{"#name":"text" "_":"Chinese Pharmacopoeia |
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